Turbo Taq Polymerase

Turbo Taq Polymerase

PfuTurbo DNA Polymerase
Reactions than occur with Taq DNA polymerase during the lower temperatures encountered during PCR cycling (e.g., primer annealing). Consequently, hot start techniques, which are commonly used with Taq … Return Doc

Pictures of Turbo Taq Polymerase

PowerPoint Presentation
Extension 68˚C or 72˚C: The polymerase copies the template adding the dNTPs from 5’ to 3’. PCR 0.1-5 ng plasmid DNA and 0.1-1 ug for genomic DNA 200 µM dNTPs (final concentration) 1-1.5 U Taq polymerase and Pfu Turbo polymerase 1.25-2.5U 0.1-1 µM of each primer fwd/rev 1-4 mM MgCl2 … Read Full Source

Pfu DNA polymerase – Wikipedia, The Free Encyclopedia
Pfu DNA polymerase is hence superior to Taq DNA polymerase for techniques that require high-fidelity DNA synthesis, but can also be used in conjunction with Taq polymerase to obtain the fidelity of Pfu with the speed of Taq polymerase activity. … Read Article

Turbo Taq Polymerase Images

HOt StARt PcR UpDAtE: CLEANAMp™ PRiMERs
The use of Turbo Primers provides at least a ten-fold increase in detection, as Primers and unmodified Taq DNA polymerase can be employed without compromising … View This Document

Turbo Taq Polymerase Images

Manual: PfuUltra(tm) High-Fidelity DNA Polymerase
Unlike Taq DNA polymerase, denaturing temperatures up to 98°C can be used successfully with PfuUltra high-fidelity DNA polymerase to amplify GC-rich regions.14, 15 … Read Content

Turbo Taq Polymerase Images

DNase I Treatment Protocol
The buffer that comes with Taq polymerase will work. Note that some commercial enzyme preparations are at 1 U/µl. 11) For critical RNA samples, consider using Ambionʼs (ABI) Turbo DNase (AM1906). … Retrieve Content

Turbo Taq Polymerase Pictures

Primers
Turbo Primers and unmodified Taq DNA polymerase gave the greatest benefit, as the amplicon yield was much higher than each of the Hot Start polymerases examined. … Get Document

Turbo Taq Polymerase Photos

Manual: PfuTurbo DNA Polymerase
Reactions than occur with Taq DNA polymerase during the lower temperatures encountered during PCR cycling (e.g., primer annealing). Consequently, hot start techniques, which are commonly used with Taq … Retrieve Full Source

Turbo Taq Polymerase Photos

Primers
Turbo and Precision Primers differ in the rate of thermal activation. CleanAmpTM Turbo Primers activate more quickly than CleanAmpTM Taq DNA polymerase (native and recombinant) Invitrogen Taq DNA polymerase (recombinant) New England Biolabs … Fetch Full Source

Images of Turbo Taq Polymerase

Manual: PfuTurbo Hotstart DNA Polymerase
Unlike Taq DNA polymerase, denaturing temperatures up to 98°C can be used successfully with PfuTurbo hotstart DNA polymerase to amplify GC-rich … View Full Source

Turbo Taq Polymerase Photos

T O POcloning PC Rcloning Fora 50m Lreactionusing Stratagene …
TOPO cloning PCR cloning For a 50 mL reaction using Stratagene Pfu Turbo DNA Polymerase (cat# 600250, blunt ends) : 1. 5 µ L 10x Reaction buffer 2 µ L dNTPs 5 mM each 1 µ L Taq DNA Polymerase 2. … Content Retrieval

Wikipedia:Reference Desk/Archives/Science/2008 May 9 …
1.10 CO2 of fans; 1.11 Sensitivity of Taq polymerase to heat Oh, and just a recommendation, regular Taq Pol is good, but I would suggest trying to use Pfu turbo or ultra. … Read Article