Topo Taq Polymerase

Topo Taq Polymerase Images

Protocol: Ligation With TOPO TA Cloning Kit (5 April 05)
• Reagents • Millipore H2O • 10X PCR buffer • dNTPs • T7 and M13r primers (1:10 dilution) • Taq polymerase • White colonies from ampicillin-selective plates (TOPO or P-GEM … Read Document

Polymerase Chain Reaction – Wikipedia, The Free Encyclopedia
A related patent battle over the Taq polymerase enzyme is still ongoing in several jurisdictions around the world between Roche and Promega. The legal arguments have extended beyond the lives of the original PCR and Taq polymerase patents, which expired on March 28, 2005. … Read Article

Topo Taq Polymerase Images

TOPO CLONING
TOPO cloning kit is available for PCR cloning with either Taq DNA polymerase or a proofreading enzyme. Some examples are. TOPO TA Cloning Kit. Zero Blunt TOPO PCR Cloning Kit … Fetch Full Source

TOPO Cloning – Wikipedia, The Free Encyclopedia
Different types of vectors are used for cloning fragments amplified by either Taq or Pfu polymerase as Taq polymerase (unlike Pfu) leaves an extra "A" nucleotide at the 3'end during amplification. TA TOPO Cloning technique relies on the ability of adenine (A) and thymine (T) (complementary basepairs … Read Article

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MacVector 12
TOPO-TA Cloning Taq polymerase has a non template-dependent activity that adds a single deoxyadenosine (A) to the 3́ ends of PCR products. This … Get Document

Topo Taq Polymerase Pictures

Troubleshooting Guide For PCR
Taq DNA Polymerase (#EP0611), Maxima ™ Hot Start Taq DNA Polymerase (#EP0601) or PyroStart Fast PCR Master Mix (2X) (#K0211). Alternativley, use enzymes or master mixes with higher sensitivity than that of Taq … Retrieve Full Source

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Chimera-Free Cloning Of Single DNA Inserts Using “GC Cloning”
Taq DNA polymerase. Taq DNAP was used with Taq DNA polymerase in the presence of dATP (A), dTTP (T), dGTP (G), or dCTP (C).After treatment with Taq poly-merase, the DNA was ligated by T4 DNA ligase. Lane Blunt) lambda/Hinc II DNA, self-ligated. M 0 A T G C Blunt GC Cloning versus TOPO TA Cloning … Get Document

TA Cloning – Wikipedia, The Free Encyclopedia
It is best if the PCR primers have guanines at the 5' end as this maximizes probability of Taq DNA polymerase adding the terminal adenosine overhang. Thermostable polymerases containing extensive 3´ to 5´ exonuclease activity should not be used as they do not leave the 3´ adenine-overhangs. … Read Article

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Easy-A High-Fidelity PCR Cloning Enzyme And Master Mix
• Easy-A-amplified PCR products are ready to clone into the TOPO TA Cloning® vector and other T-/U- vectors⇑ • High throughput method eliminates post-PCR A-addition steps and contamination risk • Cloning efficiencies comparable to Taq DNA polymeraseDNA polymerase … Fetch Doc

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Gateway Technology With Clonase II
• For rapid TOPO® Cloning of Taq polymerase-amplified PCR products, use one of the pCR ®8/GW/TOPO TA Cloning® Kits. • For directional TOPO® Cloning of PCR products, use one of the pENTR™ D- … Fetch Content

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T O POcloning PC Rcloning Fora 50m Lreactionusing Stratagene …
1 µ L Taq DNA Polymerase 2. Incubate at 72 ° C for 10 min. TOPO TA cloning (Invitrogen cat #K2500 – 20) 1. Combine the following: 4 µ L PCR product 1 µ L Salt solution 0.5 µ L TOPO vector 2. … Visit Document

Topo Taq Polymerase

23 2001 OLUME Featured Papers
If you wish to use a TOPO TA vector, you can take advantage of the terminal transferase-like activity of Taq DNA polymerase to quickly add the 3´-A … View Full Source

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TOPO-cloning Of PCR Products
TOPO-cloning of PCR products 1. Perform PCR: • if Taq was used alone, continue the rest of this protocol within the same day as the • if Pfu or another proofreading polymerase was used (alone or in combination with Taq), allow reaction to sit at room temperature (RT) or at 4˚C … Fetch Doc