Taq Polymerase Vs Phusion

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Fidelity vs. Taq 1x 2x 52x Blunt or 3’ A end 3’ A Blunt Blunt Speed 1 min/kb 10-15 s/kb 15-30 s/kb Offer Code SKU Description Size Offer F-530S-MP35 F-530S Phusion High-Fidelity DNA Polymerase … Access Doc

Z[Y`SLHKPUNÄKLSP[ ` Kits Library KAPA Amplification
The error rate of KAPA HiFi is 100X lower than Taq polymerase, 40X lower than polymerase blends and 2X lower than Phusion ¨ . For sales please contact VDOHV#NDSDELRV\VWHPV FRP or call your local representative. … Read Full Source

PCR Enzymes For Routine And High-fidelity PCR
Fidelity vs. Taq 1x 2x 52x Blunt or 3’ A end 3’ A Blunt Blunt Speed 1 min/kb 10-15 s/kb 15-30 s/kb Buy 2 Get 1 FREE Cat. No. Description Size List Price* F-530S Phusion High-Fidelity DNA Polymerase … View This Document

EnginEErEd Dn A PolymErAsEs EnAblE DEcrEAsEd AmPlificAtion …
We compared coverage bias resulting from PCR amplification of NGS libraries using a standard Taq DNA polymerase (KAPA Taq) versus a mutant 30 0.5 0.6 0.7 0.8 windowed depth vs %GC, S. aureus l_7_tru2 amp:phusion fracGC … Access Doc

ROUTINE PCR REAGENTS TO MEET ANY BUDGET AMPLIFICATION – PCR …
vs. Pfu (fold) PfuUltra ® II Fusion HS DNA Polymerase 185 12.3 Phusion ™ DNA Polymerase 30 – 35 2.3 Pfx50 polymerase, Taq DNA polymerase, and our ArchaeMaxx polymerase-enhancing factor, along with a special buffer … Access Doc

Dept. Of Agricultural Bio-resources National Academy Of …
• Fidelity: Up to 52x vs Taq, 7x vs. Pfu • Robustness: fewer reaction failures, minimal optimization Superior features of Phusion DNA Polymerase; 15-30 s/kb extension, 25x Taq fidelity, amplifies GC-rich amplicons, tolerates … Read Document

KAPA NGS Catalog (11 2011)
Is 100X lower than Taq polymerase, 40X lower than polymerase blends and 2X lower than Phusion .® 6 -7 2.50 E-5 2.00 E-5 1.50 E-5 1.00 E-5 0.50 E-5 0.00 E-5 … Access Full Source

Direct PCR From A Single Bacterial Colony Without DNA …
Colony using FailSafe and the leading Taq DNA Polymerase All PCR amplifications using the FailSafe System were performed as indicated above. PCR amplifications using the … Fetch Document

PCR And RT-PCR Reagents AMPLIFICATION
vs. Pfu (fold) PfuUltra® II Fusion HS DNA Polymerase 185 12.3 Phusion™ DNA Polymerase 30 – 35 2.3 Phusion ™and iProof DNA polymerases and 20-fold higher than Taq DNA Polymerase. … Fetch Document

Pfu DNA polymerase – Wikipedia, The Free Encyclopedia
Pfu DNA polymerase is hence superior to Taq DNA polymerase for techniques that require high-fidelity DNA synthesis, but can also be used in conjunction with Taq polymerase to obtain the fidelity of Pfu with the speed of Taq polymerase activity. … Read Article

A Scientific Update From New England Biolabs
Phusion High-Fidelity DNA Polymerase* 50X ≤ 20 kb Blunt Yes Yes/Yes 0.5–1.0 Optimal PCR Kit/ Fidelity Extension Difficult Resulting 3´ 5´ Master Mix Units/50 µl vs. Taq Range Templates DNA ends exo Available Reaction Advantages … Fetch Full Source