Taq Polymerase Specific Activity

Images of Taq Polymerase Specific Activity

Platinum Taq DNA Polymerase
Taq DNA Polymerase (EC 2.7.7.7) is a 832 amino acid single subunit enzyme with a MW of 94,000 [Lawyer (1989) JBC 264, 6427] and a specific activity of 100,000 U/mg of pure protein with one unit corresponding to 50 fmol of … Get Content Here

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Polymerase Chain Reaction Optimization – Wikipedia, The Free …
Taq polymerase lacks a 3' to 5' exonuclease activity. Thus, Taq has no error-proof-reading activity, which consists of lower concentrations of magnesium or other cations may prevent non-specific primer interactions, thus enabling successful PCR. A "hot-start" polymerase enzyme whose activity is … Read Article

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For General Laboratory Use. Not For Use In Diagnotic …
• Template DNA, gene-specific PCR primer pair • dNTPs, PCR Grade* • Water, PCR Grade* • Polymerase Chain Reaction (PCR): Taq DNA Polymerase activity is stable during prolonged incubation at high temperatures (95°C) … Read Here

Taq Polymerase Specific Activity Photos

Taq PCR Handbook
The reactions must yield a single specific product. Exonuclease activity assay: Linearized DNA is incubated with Taq DNA Polymerase in QIAGEN PCR Buffer. … Read Here

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TrueStart™ Hot Start Taq DNA Polymerase
Assayed for9700 polymerase activity after the incubation at 95°C in the reaction buffer Specific amplification of a 2 kb fragment from the human β-globine gene using Maxima™ Hot Start Taq DNA Polymerase … Read Document

Taq Polymerase Specific Activity Images

HE IOLOGICAL Printed In U.S.A. Structure-specific DNA-induced …
Nism proposed for nuclease activity (9). Biochemical studies have shown that the 5 -nuclease of Taq polymerase recognizes and endonucleolytically cleaves a struc-ture-specific DNA substrate that has a bifurcated downstream … Fetch Here

Taq Polymerase Specific Activity Pictures

AccuPrime Taq DNA Polymerase High Fidelity Cat. Nos. Size …
Antibody complexes with Taq DNA polymerase and inhibits activity at room temperature. Activity is restored after the initial denaturation step at 94ºC, providing an automatic “hot start” PCR. 4,5 The thermostable AccuPrime ™ protein enhances specific primer-template hybridization … Fetch This Document

Taq Polymerase Specific Activity Photos

Variants Of PCR – Wikipedia, The Free Encyclopedia
It is lacking 5'-3' exonuclease activity, and may be able to amplify longer targets than the native enzyme. Faststart polymerase is a variant of Taq polymerase that requires strong heat activation, thereby avoiding non-specific amplification due to polymerase activity at low temperature (see hot-start … Read Article

Taq Polymerase Specific Activity Pictures

Acad. Sci. USA
The 5' -* 3' exonuclease activity of Taq DNA polymerase degrades the probe only when the probe is hybridized to specific target. Evenin ahigh background of … View Doc

Images of Taq Polymerase Specific Activity

POLYMERASE CHAIN REACTION (PCR)
Required for specific primer template interactions is affected by Mg++ concentrations; phosphate buffers and excess EDTA inhibit the activity of Taq polymerase by affecting the Mg++ … Retrieve Document

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TaqMan – Wikipedia, The Free Encyclopedia
The TaqMan probe principle relies on the 5´–3´ exonuclease activity of Taq polymerase to cleave a dual probes are designed such that they anneal within a DNA region amplified by a specific set of primers. (Unlike the diagram, the probe binds to single stranded DNA.) As the Taq polymerase … Read Article

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AccuStart Taq DNA Polymerase
The AccuStart automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. Activated AccuStart Taq DNA polymerase possesses 5’!3’ DNA polymerase activity and a double-strand specific 5’!3 … Fetch Full Source

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HotStarTaq PlusPCR Handbook
HotStarTaq PlusDNA Polymerase HotStarTaq PlusMaster Mix Kit For highly specific hot-start PCR 94 kDa Taq DNA Polymerase from QIAGEN. HotStarTaq Plus DNA Polymerase is provided in an inactive state with no polymerase activity at ambient temperatures. … View This Document

MDobbelstein – YouTube
Here, the reasons for non-specific PCR products are covered. As a solution, it is suggested to use touchdown PCR, a special design of taqman, the fluorescence signal is based on a the cleavage of a fluorescently labelled oligonucleotide (through exonuclease activity of Taq polymerase). … View Video

Factors For Successful PCR – Polymerase Chain Reaction – PCR …
New primers with a positive control reaction, to be sure they work under your specific Reaction Mix: Enzyme Activity The choice of DNA polymerase affects the fidelity of the reaction and quality of product. … Read Article