Taq Polymerase Overhangs

CloneJET PCR Cloning Kit #K1231, #K1232
• Use for cloning PCR products with 3'-dA overhangs generated by Taq DNA polymerase, DreamTaq™ DNA polymerase or enzyme mixtures containing Taq DNA polymerase. … View Doc

Taq DNA Polymerase DNA-free
Taq DNA Polymerase DNA-free From Thermus aquaticus BM, recombinant (E.coli) Deoxynucleoside-triphosphate: DNA deoxynucleotidyltransferase, Taq Pol, EC 2.7.7 1.5 mM when using 200 μM dNTP each PCR product size optimized for up to 5 kb PCR cloning TA-cloning; addition of A-overhangs ("extendase activity") … Read Full Source

STGN Polymerase Selection Guide
Proofreading DNA polymerase which adds 3’ -A overhangs to PCR amplicons. Combines accuracy of Pfu with TA/UA cloning effi ciency of Taq.  (6x Taq) … View Doc

Blunt Ending DNA Fragments
We have currently two choices for this procedure. T4 DNA polymerase or Mung bean nuclease. T4 polymerase will blunt DNA by filling in 5’ overhangs using its 5’-3’ polymerase activity, or clipping off 3’ overhangs with its 3’-5’ exonuclease. … Access Content

Taq DNA polymerase And A 2X Description
Elongation rate 2x higher than regular Taq DNA polymerase, and can shorten the amplification time by half. It has 5' to 3' polymerase activity but lack of 3' to 5' exonuclease activity, that results in a 3'-dA overhangs PCR product. … Read Full Source

FS Taq Mix Direct For Blood
Elongation rate 2x higher than regular Taq DNA polymerase, and can shorten the amplification time by half. It has 5' to 3' polymerase activity but lack of 3' to 5' exonuclease activity, that results in a 3'-dA overhangs PCR product. … Access Doc

Photos of Taq Polymerase Overhangs

Subcloning Notebook, BR152
A-overhangs created by the Taq DNA polymerase. Start with 1–7µl of purified PCR fragment generated by a proofreading polymerase (e.g., Pfu DNA Polymerase). … Retrieve Here

Myocyte Death In The Failing Human Heart Is Gender Dependent
Single-base 39 overhangs are identified by a polymerase chain reaction–generated Taq polymerase probe that possesses comple mentary structures (Figure 1A). … Read More

Restriction Enzymes – All About Restriction Enzymes
The former cut will generate "blunt ends" with no nucleotide overhangs. The latter, generates "sticky" or "cohesive" ends, because each resulting Polymerase Chain Reaction – PCR – how pcr works; DNA Sequencing – DNA Analysis – Methods for Sequencing DNA … Read Article

Klenow Fragment – Wikipedia, The Free Encyclopedia
Digesting away protruding 3' overhangs; Preparation of radioactive DNA probes; The Klenow fragment was also the original enzyme used for greatly amplifying segments of DNA in the polymerase chain reaction (PCR) process, before being replaced by thermostable enzymes such as Taq polymerase. … Read Article

Taq Polymerase Overhangs Pictures

Taq polymerase – Wikipedia, The Free Encyclopedia
Taq polymerase ( / ˌ t æ k ˈ p ɒ l ɨ m ər eɪ z /) is a bacteria and archaea, such as Pfu DNA polymerase, possessing a proofreading activity, and are being used instead of (or in combination with) Taq for high-fidelity amplification. Taq makes DNA products that have A overhangs at their 3' ends. … Read Article

Roche Applied Scienc – 1. Introduction
The ends of PCR products produced with Taq enzymes can be pol ished using T4 DNA polymerase to remove the 3’-A overhangs if blunt-end cloning is intended. … Fetch Full Source

Easy-A High-Fidelity PCR Cloning Enzyme And Master Mix
Easy-A™ High-Fidelity PCR Cloning Enzyme and Master Mix • Proofreading DNA polymerase formulation that adds 3’-A overhangs to PCR amplicons as efficiently as Taq … Fetch This Document