Taq Polymerase Nuclease

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Real-Time PCR Vs. Traditional PCR
The 5 nuclease assay uses this activity in real time detection. Figure 9: Taq polymerase activity Figure 10: 5 Exo-Nuclease Activity of Taq Polymerase: … Access Document

SNP Genotyping – Wikipedia, The Free Encyclopedia
Taq DNA polymerase’s 5’-nuclease activity is used in the TaqMan assay for SNP genotyping. The TaqMan assay is performed concurrently with a PCR reaction and the results can be read in real-time as the PCR reaction proceeds (McGuigan & Ralston 2002). … Read Article

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Taq PCR Handbook
Nuclease contamination: No Protease contamination: No RNase contamination: No Taq DNA Polymerase Kit 100 x 250 units Taq DNA 201209 (25000 U) Polymerase, 10x PCR Buffer,* … Fetch Document

Taq Polymerase Nuclease

Certificate Of Analysis Flexi DNA Polymerase: GoTaq Part …
Nuclease Assays: No contaminating endonuclease or exonuclease activity detected. GoTaq Flexi DNA Polymerase Product Information Sheet Keywords: pcr, taq, magnesium optimization … Get Document

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Taq DNA Polymerase (recombinant)
Taq DNA Polymerase 1.25 u Water, nuclease -free (#R0581) to 50 µl Total volume 50 µl *Volumes of 25 mM MgCl2, required for specific final MgCl2 … Read Full Source

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JumpStart Taq DNA Polymerase (D9307) – Technical Bulletin
JumpStart™ Taq DNA Polymerase With 10× reaction buffer containing 15 mM MgCl2 5’ Nuclease and dsDNA-Binding Dye Processes. For further information contact t he Director of Licensing, … Read Full Source

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Choose The Best Enzyme For Your Application
As the patented 5’ Nuclease Process claims in US Patents Nos. 5,210,015 and 5,487,972), no right to perform any patented method, and no right GoldStar® DNA polymerase is a recombinant Taq DNA polymerase that is isolated from extremely thermostabile … Retrieve Content

Taq Polymerase Nuclease Pictures

UNIVERSITY OF WESTMINSTER School Of Life Sciences Polymerase
Note that Taq polymerase, the most commonly used enzyme has no proof reading ability (no 3’-5’ nuclease activity). Primers, typically 20 – 30 oligonucleotides long are required to initiate extension … Access Full Source

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STRONG Taq DNA Polymerase
• Recombinant Taq DNA polymerase (5 U/μl.) • 10X PCR Buffer with 15 mM MgCl2, 1.3 ml by testing each nuclease assay in PCR buffer. ※Note: 1. Avoid repeated freeze-thaw cycles of both the enzyme and the … Fetch This Document

TaqMan – Wikipedia, The Free Encyclopedia
The TaqMan probe principle relies on the 5´–3´ exonuclease activity of Taq polymerase to cleave a dual-labeled probe during hybridization to the complementary target sequence and fluorophore-based detection. As in other real-time PCR methods, the resulting fluorescence signal permits quantitative … Read Article

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AccuStart Taq DNA Polymerase HiFi
Nuclease-free water variable HiFi PCR Buffer (10X) 5 µL 1x 50 mM magnesium sulfate 2 µL 2 mM 10 mM dNTP Mix 1 µL 200 µM each dNTP Forward primer variable 100 – 500 nM Reverse primer variable 100 – 500 nM AccuStart Taq DNA Polymerase HiFi 0.2 µL 1 AccuStart Taq DNA Polymerase … Access Full Source

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Klear Taq DNA polymerase
Bacterium Thermus aquaticus, obtained by high-level expression of the Taq DNA polymerase gene in E. coli. activity but lacks a 3” – 5’ nuclease activity and therefore does not have a … Access Doc