Taq Polymerase Mutation Rate

PCR Amplification Introduces Errors Into Mononucleotide And …
Of simple polymorphism or mutation (table 1). The sequencing of more than 30 individual electrophoresis comparing the Taq polymerase amplified alone would have on polymerase error rate. Our data lead to the conclusion that polymerase per- … Document Retrieval

The Nucleotide Targets Of Somatic Mutation And The Role Of …
The overall mutation rate of A nucleotides is not significantly different from that expected by sequence-insensitive mutations, and a matic mutation or to Taq polymerase errors that arose during PCR amplification. … Document Retrieval

Removal Of polymerase-produced mutant Sequences From PCR Products
Contain a mutation somewhere in their sequence,l is the length of the amplified sequence in base pairs, n is the number of cycles, and a is the error rate for the polymerase expressed per nucleotide incorporated. Because the error rate for Taq DNA … Get Content Here

II EZClone Domain Mutagenesis Kit
Developed Mutazyme II DNA polymerase to over come these biases and produce a more uniform mutation spectrum. the other hand, Taq polymerase under error-prone conditions and This result is consistent with the error rate of Mutazyme II DNA polymerase listed in the kit … Fetch Document

Pfu DNA polymerase – Wikipedia, The Free Encyclopedia
Commercially available Pfu typically results in an error rate of 1 in 1.3 million base pairs and can yield 2.6% mutated products when Pfu DNA polymerase is hence superior to Taq DNA polymerase for techniques that require high-fidelity DNA synthesis, but can also be used in conjunction with Taq … Read Article

7.28 Spring ‘01 Problem Set #1 Answers
If Taq polymerase had a processivity of 100 bp: 1000/100=10 On average the Taq enzyme would only have to find the primer template junction 10 times Z_____ OR _____+_____ _____+_____ c) How would the following mutations affect the mutation rate of E. coli? … Retrieve Full Source

MutS, Mutation In DNA: Application
DNA(250 ng) was amplified with Taq polymerase to ofheterozygosity forR553Xpoint mutation. GenomicDNAwasamplified withPfuor Taqpolymerase; higher misincorporation rate than thermolabile DNApolymerases(14). ThefidelityofPfuDNApolymerase is reported to be 12-fold higher than the fidelity of Taq … Document Viewer

Investigating The Effects Of DMSO On PCR Fidelity Using A …
Taq polymerase (Taq), which is commonly used in PCR, possesses no proof-reading ability and has an effect DMSO may have on the fidelity of Taq, in which proper base-pairing is dependent on the formation of correct hydrogen bonds. Again, mutation rate could be … Fetch This Document

High-Fidelity In Vitro Recombination Using A Proofreading …
Proofreading Polymerase BioTechniques 30:530-536 (March 2001) ABSTRACT We describe a convenient PCR-based the mutation rate of a modified StEP Short Technical Reports 532BioTechniques Vol. 30, No. 3 (2001) DNA Amino Acid … Access Doc

Manual: PfuUltra Hotstart DNA Polymerase
KOD DNA polymerase 3.5 × 10–6 7.0 Taq DNA polymerase 8.0 × 10–6 16.0 a Fidelity is measured using a published PCR forward mutation assay that is based on the lacI target gene.2 b The error rate equals mutation frequency per base pair per duplication. … View This Document

Biochimica Et Biophysica Acta
With Taq polymerase. To increase mutation rate, product of the first amplification was used as a template in the next PCR reaction. Re-amplification was repeated four times. … Doc Viewer

Taq polymerase – Wikipedia, The Free Encyclopedia
It lacks a 3' to 5' exonuclease proofreading activity, and has an error rate measured at about 1 in 9,000 nucleotides. The remaining two domains In December 1999, U.S. District Judge Vaughn Walker ruled that the 1990 patent involving Taq polymerase was issued, in part, on misleading information and … Read Article