Taq Polymerase Mg2

Taq Polymerase Mg2

X G ENAXXON – SuperHot Taq-DNA-Polymerase
SuperHot Taq-Polymerase is shipped on “blue-ice” but retain full activity at RT (15-25°C) for at least 2 weeks. Note: The optimal Mg2+ concentration should be determined empirically but in most cases a concentration of 2.5mM, as provided in the … Access Full Source

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PCR Enhancers And Additives
Taq DNA polymerase is isolated from Thermus aquaticus and catalyzes the primer-dependent incorporation of nucleotides into duplex DNA in the 5′→3′ direction in the presence of Mg2+. … Read More

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Polymerase Chain Reaction (PCR)
Taq polymerase: 0.5 – 1.0 Units/50ul reactions Protocol for PCR cocktail 1. – 0.10% v/v) Higher than 50mM KCl or NaCl inhibits Taq, but some is necessary to facilitate primer annealing. [Mg2+] affects primer annealing; Tm of template, product and … Doc Viewer

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Certificate Of Analysis Taq-Pro Core Kit
Taq-Pro DNA Polymerase 500u 10x NH 4 Buffer 2 x 1.2ml MgCl 2 (50mM) Solution 1.2ml Mg2+ Stock solution: 50mM MgCl2 (suggested final concentration 1.5mM-4mM). … Return Document

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PowerQ® Taq DNA Polymerase
PowerQ® Taq DNA Polymerase for qPCR User Manual Storage condition: The undiluted enzyme solution is stable when stored 10×Reaction buffer (without Mg2+ ): 100mM Tris-HCl; 500mM KCl; pH 9.0 (20℃). … Retrieve Content

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Troubleshooting Guide For PCR
Taq DNA Polymerase (#EP0611), Maxima ™ Hot Start Taq DNA Polymerase (#EP0601) or PyroStart Fast PCR Master Mix (2X) (#K0211). Alternativley, use enzymes or master mixes with higher sensitivity than that of Taq … Read Here

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Axygen Taq Polymerase Kit No Bacterial Chromosomal DNA …
Reagents Supplied in the Axygen Taq polymerase kit – Standard Taq Polymerase -500 units. – Taq Reaction Buffer -1ml – Taq Reaction Buffer-Mg2 Free -1ml … Access Content

Polymerase Chain Reaction Optimization – Wikipedia, The Free …
Magnesium is required as a co-factor for thermostable DNA polymerase. Taq polymerase is a magnesium-dependent enzyme and determining the optimum concentration to use is critical to the success of the PCR reaction. Some of the components of the reaction mixture such as template concentration, dNTPs … Read Article

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GoTaq Amplification Family Tree (amplify Trees)
To Start a GoTaq Buffer Info Experience higher performance in your standard PCR reactions GoTaq Polymerase gives higher yields than standard Taq DNA Polymerase Reactions assembled with either 1.25u of GoTaq DNA Polymerase or 1.25u of standard Taq DNA polymerase. All reactions used 1.5mM MgCl2. Mg2 … Fetch This Document

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PowerPoint Presentation
Sometimes) dNTPs Taq polymerase Primers DNA template Buffer + + A C T G MgCl2 MgCl2 (mM) 1.5 2 3 4 5 Magnesium Chloride (MgCl2 – usually 0.5-5.0mM) Magnesium ions have a variety of effects Mg2+ acts as cofactor for Taq polymerase Required for Taq to function Mg2 … Access Document

Taq Polimerase – Wikipedia Bahasa Indonesia, Ensiklopedia Bebas
Taq polimerase (disingkat Taq pol) merupakan enzim DNA polimerase (EC 2.7.7.7) stabil suhu pertama yang berhasil dipurifikasi dari bakteri termofil ekstrem enzim ini memerlukan keempat deoksiribonukleotida dan DNA timus anak sapi yang diaktivasi. Selain itu, dibutuhkan juga kofaktor kation divalen yaitu Mg2+ … Read Article

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TaKaRa Ex Taq ™ (Mg2+ Free Buffer)
Applications: For DNA amplification by Polymerase Chain Reaction (PCR). PCR products : As most PCR products amplified with TaKaRa Ex Taq have TaKaRa Ex Taq ™ (Mg2+ free Buffer) Author: TaKaRa bio Inc. Created Date … View Full Source