Taq Polymerase Mg Concentration

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Taq PCR Handbook
However, in some cases, reactions may be improved by increasing the final Mg 2+ concentration according to Table 2. Taq DNA Polymerase Protocol … Retrieve Full Source

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For General Laboratory Use. Not For Use In Diagnotic …
In most cases a Mg2+ concentration of 1.5 mM will produce satis-factory results (2, 3) if you use 200 M of each dNTP. 6 Taq polymerase: increased enzyme versatility in DNA sequencing (1988) Applied Biosystems. … Access Content

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Choice Taq™ Blue DNA Polymerase
Between 65-75°C; Optimized for use with dNTP concentration at 100-300 µM, 1.5-3.0mM Mg 2+ concentration, and pH at 8.1-9.2. Choice Taq Blue differs in that it has a blue dye added to the enzyme. This is an aid to identify which reactions have already had Taq Polymerase added. … View This Document

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Taq DNA Polymerase
Taq DNA Polymerase – 10x NH 4 Reaction Buffer including 15 mM MgCl 2 – Concentration 5 Mg2+ and hence should be pre-purified. Template concentration will have a profound effect on PCR performance. Recommended amounts for standard PCR and reaction volume 25 µl are: – 1 – Taq DNA Polymerase (inkl. … Read Full Source

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FS Taq DNA Polymerase TM FS
FSTM Taq DNA Polymerase #P1073 1000U Lot: Concentration:5U/ l Contents:FSTM DNA Polymerase 200 l 10xFSTM PCR Buffer(Mg2+ plus) 2×1.4ml 6x Loading Buffer 1ml … Fetch Full Source

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28093 Taq 200 Unit
With 10X Mg-free Taq DNA Polymerase Taq Reaction Buffer (1000 units/mL) Catalogue Number: 28093 TaqWith 10X Mg-free Catalogue Number: 28093 Taq DNA Polymerase: A concentration of 0.02 units/µL reaction Taq Diluent (#28091) sho uld be used if … Read Here

Taq Polymerase Mg Concentration Images

Taq DNA Polymerase
• Optimal Mg concentration can vary from 1.5 mM to 5 mM; in most cases a Mg2+ concentration of Enzyme concentration too low • Increase enzyme concentration to 2 U Taq DNA Polymerase per 50 µl reaction. … Get Content Here

Gel Electrophoresis – Wikipedia, The Free Encyclopedia
Protein methods; Molecular biology; Laboratory techniques; Electrophoresis; Polymerase chain reaction … Read Article

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28087 Taq 1000 Unit
TaqWith 10X DNA Polymerase Taq Reaction Buffer (Mg-Free) (5000 units/mL) Catalogue Number: 28087 Taq DNA Polymerase: A concentration of 0.02 units/µL reaction Taq Diluent (#28091) should be used if … Access Full Source

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PCR Core Systems Taq DNA Polymerase Pfu
Containing a fixed concentration of Mg 2+ (giving a final concentration of 1.5mM in the final reaction). Most Taq DNA polymerase amplifications work well at this Mg … View Document

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POLYMERASE CHAIN REACTION (PCR)
This is accomplished by preparing a series of reaction s containing 1.5-3 mM Mg2+, in increments of 0.5 mM, by adding 3, 4, 5 and 6 µl of a 25 mM MgCl 2 stock to 50 µl reactions. C. Enzyme Concentration It is recommended that 1.25 units of Taq DNA Polymerase be used per 50 µl reaction. … Document Retrieval