Taq Polymerase Half Life

Taq DNA Polymerase
1.25 U EURx Taq DNA Polymerase in 50 µl reaction volume. Description: > Taq DNA Polymeraseis a thermostable enzyme of approximately 94 kDa from Thermus aquaticus. > Ultra pure, recombinant protein. > The enzyme replicates DNA at 74°C and exhibits a half-life of 40 min … Document Retrieval

Taq Polymerase Half Life Pictures

HotStarTaq PlusPCR Handbook
Half-life: 10 min at 97°C; 60 min at 94°C 5'–3' exonuclease activity: Yes HotStarTaq Plus DNA Polymerase is a modified form of the recombinant 94 kDa Taq DNA Polymerase from QIAGEN. … Get Content Here

I Description …
For long DNA fragment amplification, where longer elongation time is needed (in this case, the elongation time was three minutes), the green Taq DNA polymerase has a longer enzyme half-life and therefore increases the PCR DNA yield significantly. … Fetch Document

LA Taq – Standard PCR Reaction Conditions
• The half life at 95°C is 35 min, and at 97.5°C is 7 min. • Temperature range for extension is 60-72°C. Takara LA Taq™ DNA Polymerase (5 U/µL) 0.5 µL 2.5 U/50 µL 10X LA Buffer II (25 mM Mg2+ plus) 5.0 µL 1X … Document Retrieval

Ref : N B – 03 – 0119 250 U N B – 03 – 0120 250 U N B – 03 …
Features • Fast rate of elongation: elongation velocity can reach to 3 kb/min, 2x higher than regular Taq DNA polymerase • Highly thermostable : have a halflife of over 40 min at 95°C incubation • Generates 3' – dA overhangs PCR products … Fetch This Document

Taq Plus DNA Polymerase
• The half-life of enzyme is >40 minutes at 95°C. • The error rate of Taq Plus DNA Polymerase in PCR is about 1×10-5 errors per nt per cycle; the accuracy (an inverse of error rate) an average number of correct nucleotides … Return Document

X G ENAXXON – SuperHot Taq-DNA-Polymerase
SuperHot Taq-DNA-Polymerase Deoxynucleosidetriphosphate: DNA nucleotidyltransferase from Thermus aquaticus Half-life: 10min. at 97°C, 60min. at 95°C 5’-3’ exonuclease activity: Yes … View This Document

Wikipedia:Reference Desk Archive/Science/2006 July 13 …
Therefore, if your life is nothing more than staying alive, then indeed you don't need to worry About half a million people get killed by cars (directly) every year, at an average age of around And the infamous T. aquaticus, from which scientists get Taq polymerase, grows in the boiling hotsprings … Read Article

Photos of Taq Polymerase Half Life

Master Taq Manual
half-life) and activity of Taq DNA polymerase by stabilizing the enzyme during PCR. TaqMaster makes the enzyme less sensitive to exogenous PCR-inhibiting … Access Full Source

Potato Virus Y – Wikipedia, The Free Encyclopedia
Colonizing aphids are better adapted to life on potato plants and are thus regions flanking the target DNA provide 3’-hydroxyl groups for DNA polymerase catalyzed extension. The most commonly used DNA polymerase is Taq, a of polymerases which are more thermal stable and have longer half-lives. … Read Article

Enzyme Biotechnology In Everyday LifeAbout.com Biotech And …
Enzyme Biotechnology in Everyday Life. From Theresa Phillips, former About.com Guide Since the early half of this decade, esterases have become a common approach to stickies control. … Read Article

Taq Polymerase Half Life Photos

MasterTaq Kit
TaqMaster is a buffer additive that improves thermostability (enzyme half-life) and processivity of Taq DNA polymerase by stabilizing the enzyme during PCR. DNA at 37°C in the presence of 15-20 units of Taq DNA Polymerase. The recombinant Eppendorf Taq DNA Polymerase in the MasterTaq Kit … Read Document

Polymerase Chain Reaction (PCR)
Taq polymerase is given as having a half-life of 30 min at 95 . o C, which is partly why one should not do more than about 30 amplification cycles: however, it is possible to reduce … View This Document