Taq Polymerase Half Life Room Temperature

Wikipedia:Reference Desk/Archives/Science/2008 March 2 …
To 0 half, I was If Taq polymerase is good to go at 95 C, why does it need to be stored at -20? How quickly will it break down at room temperature?Seans long shelf life. Taq poly is thermostable, which means it can operate at 95 degrees, but that's not the way to preserve it. At room temperature … Read Article

I Description …
Month when stored at room temperature (25°C) without significant activity loss.  High PCR yield: The green Taq DNA polymerase has longer enzyme half-life and therefore … Retrieve Content

Perpetual Taq DNA Master Mix (2x)
> Formation of complexes between Taq DNA Polymerase and an anti-Taq antibody forms a basis for hot start PCR, which allows for convenient room-temperature > Perpetual Taq DNA Polymerase replicates DNA at 72°C and exhibits a half-life of … Access Doc

X G ENAXXON – SuperHot Taq-DNA-Polymerase
Taq polymerase is a modified form of thermostable Taq DNA polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Half-life: 10min. at 97°C, 60min. at 95°C 5’-3’ exonuclease … Access Full Source

Focus 21.2 P.46 PLATINUM Pfx DNA Polymerase For High-Fidelity PCR
Facilitates high throughput and room temper-ature reaction setup. W hile Taq DNA polymerase is the reactivation occurred when the temperature reached 94°C. The half-life for PfxDNA polymerase is 12 h at 95°C (1). … Fetch Document

Clontech Laboratories, Inc. Titanium Taq PCR Kits User Manual
Titanium Taq DNA Polymerase is a highly sensitive, robust enzyme for use in all PCR • An integrated hot start antibody allows room temperature reaction set-up and increased Half-Life: The half-life of Titanium Taq depends on the specific reaction conditions used, but generally … Retrieve Content

> QPCR And QRT-PCR Performance
• Platinum® Taq hot-start technology for improved qPCR specificity and sensitivity • SuperScript™ III Reverse Transcriptase (RT) with increased thermostability and a longer half-life with room temperature shipping and storage • Higher specificity from Platinum® Taq DNA Polymerase … Return Doc

High-level Expression, Purification, And
Length Taq Pol I has an activity half- life of 9 min at 97.5~ The at 70~ for 10 min, and cooled to room temperature. half-life of about 2 hr at 100~ Taq Pol I has many features in com- mon with E. coli DNA polymerase I. Con- … Retrieve Document

Taq Full DNA Polymerase
Taq Full DNA Polymerase is a high-quality, full-length recombinant version of Its half-life of 40 minutes at 95°C makes it highly suitable for lengthy PCR cycling Reactions were assembled at room temperature and performed using … Get Doc

High-performance, Real-time PCR From The Ground Up
• Platinum® Taq DNA Polymerase with antibody-mediated hot-start technology for higher PCR specificity and yield with room temperature setup • SuperScript™ III Reverse Transcriptase with increased thermostability and half-life for higher cDNA yields and bet- … View Document

POLYMERASE CHAIN REACTION
10 mM Tris pH 8.3 -8.8 (at room temperature) ~ 7.0 -7.5 at 72 oC (optimal pH for Taq) 3) DNA POLYMERASE Taqis almost universally used (due to its thermostability, reliability and efficiency). Thermostable, half-life > 2 h at 92.5 oC … View Document