Taq Polymerase Genecraft

AND ORBICULE MORPHOLOGY Belg. J. Bot. 141 – THECIACEAE …
Each PCR reaction (25 µl) contained 5 µL MilliQ H 2 0, 2.5 µL 10x PCR Buffer (Genecraft, Lüdinghausen, Germany), 5 µL forward primer (2.2 µM), 5 µL reverse primer (2.2 µM), 2.5 µL dNTP (10 mM), 0.2 µL BioTerm Taq polymerase (5U µL -1 ) (Genecraft) and 5 µL template DNA (1 … Fetch Content

Assessment Of Genetic Diversity In Triticum Spp. And Aegilops …
0.25 mM dNTPs, 10 x PCR buffer, 1.2 mM MgCl2 and 1 U Taq Polymerase (Accutherm, GeneCraft, Germany). Samples were subjected to pre-amplification thermocycle profile [initial denature- … Fetch Content

Application Of Denaturing High-Performance Liquid …
2 (2 mM), 1 PCR buffer, 0.625 U of Taq DNA polymerase (BioTherm; GeneCraft), and 1 l of template for environmental samples and up to 3 Clone PCR was carried out according to standard protocols. … Retrieve Content

Evolutionary History Of The Endemic Lake Tanganyika Cichlid …
(15 mM) and 0.2 ll TAQ polymerase (5 U ll)1; Biotherm, Genecraft). The PCR cycle conditions started with a denaturation phase (94 C, 3 min) followed by 45 cycles of 94 C, 30 s; 50–54 C, 30 s; 72 C, 30 s. … Retrieve Doc

A SINE Of Restricted Gene Flow Across The Alpine Fault …
BioTherm DNA Polymerase (GeneCraft), or (ii) 1 × reaction buffer (20 m m Tris-HCl (pH 8.4), 50 m m (KCl), 1.5 m m MgCl 2 and 1 U of Platinum Taq DNA Polymerase (Invitrogen). … Visit Document

Phylogeography Of The Spotted Skink (
And 1 U of BioTherm DNA Polymerase (GeneCraft), or in 25 lL volumes containing 1 lL of DNA template, 1· reaction buffer (20 mM Tris–HCl (pH 8.4), 50 mM KCl), … Get Doc

Nicotinamide N-Methyltransferase Upregulation Inversely …
25 µL with Taq Polymerase and buffers purchased from GeneCraft (Luding-hausen, Germany). Gene-specific PCR primers for human NNMT and β-actin were designed, based on the DNA se- … Retrieve Document

Interaction Between Pro-inflammatoryand Anti-inflammatory …
And Biotherm Taq polymerase from GeneCraft (Mu¨nster, Germany).TheSuperScriptIIRTreversetranscriptaseandall tissuecultureequipmentwerepurchasedfromInvitrogen.All … View Doc

Effects Of The Novel Mitochondrial Protein Mimitin In Insulin …
Germany), BiothermTM Taq polymerase from GeneCraft (Münster, Germany). The SuperScript II RTTM reverse transcriptase and all tissue culture equipment were from … Retrieve Content

Visualization Of Endogenous P53-Mediated Transcription In …
Deoxynucleotide triphosphate (1 AL), and Taq-DNA polymerase (2.5 units, GeneCraft, Mu¨nster, Germany) using a GeneAmp PCR system (Applied Biosystems, Foster City, CA). … Access Doc