Taq Polymerase Gc Rich

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DNA Polymerases
When amplifying difficult templates containing GC-rich or long complementary areas, PCR additives such as DMSO are often DyNAzyme™ EXT DNA Polymerase Taq Taq-based mix min Half-lifes lives of DNA polymerases in the presence of 5 % DMSO. … Return Doc

Taq Polymerase Gc Rich

BIO VIEW
Features • Highest level of accuracy for PCR. • Highly efficient amplification exceeds that of Taq DNA polymerase. • GC-rich sequences are amplified efficiently and … Fetch This Document

Taq Polymerase Gc Rich Photos

MiL Hot Start Taq DNA Polymerase
• Difficult templates e.g. secondary structures or GC-rich sequences. • Cloning with TA and blunt ends. KIT CONTENTS. Content Volume Hot Start Taq DNA Polymerase (5U/ uL) 100 uL (500U) 10X PCR buffer (w/ 20mM Mg2+) … View This Document

Taq Polymerase Gc Rich Photos

High Activity Taq Polymerase
Phenix Taq DNA Polymerase can amplify fragments from genomic DNA of up to 6Kb. Specificity and performance of Taq can be further improved with the use of 2x PolyMate Additive (not supplied, see associated products), which is designed for GC or AT rich DNA, “dirty” templates or sequences with … Read Here

Taq Polymerase Gc Rich Pictures

It’s Time For 5 PRIME – The New Dimension In PCR
Hot-start PCR, classical reaction set-up HotMaster Taq DNA Polymerase Hot-start PCR, convenient reaction set-up 5 PRIME HotMasterMix Special PCR Application Choice of enzyme Multiplex PCR (> 2 products) HotMaster Taq Polymerase GC-rich and contamined samples MasterTaq Kit … Return Document

Nucleic Acid Thermodynamics – Wikipedia, The Free Encyclopedia
The process of DNA melting is also used in molecular biology techniques, notably in the polymerase chain ΔG° 37 (predicted) = ΔG° 37 (CG initiation) + ΔG° 37 (CG/GC) + ΔG° 37 (GT/CA) + ΔG° 37 (TT/AA http://www.promega.com/biomath/calc11.htm#disc; by Alexander Rich … Read Article

Taq Polymerase Gc Rich Pictures

Robust PCR Amplification Of GC-rich Targets With Hot Start 7 …
DNA and Taq DNA polymerase. Figure 2. Detection of three targets with ~60% GC content using the CleanAmp™ 7-deaza- lutions for the amplification of GC-rich regions, none are optimal and therefore may require … Retrieve Content

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Non-radioactive Fragile X CGG Repeat Detection By PCR*
10% DMSO can reduce Taq polymerase activity by up to 50% so it should not be used routinely. Formamide Reduces secondary structure and is particularly useful for GC rich templates. … Doc Viewer

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Taq DNA Polymerase (recombinant)
Taq DNA Polymerase should be added after the initial denaturation step to avoid a decrease in its activity. Denaturation A DNA denaturation time of 30 seconds per cycle at 95°C is normally sufficient. For GC-rich DNA templates, this step … View Document

Thermus Thermophilus – Wikipedia, The Free Encyclopedia
Halobacterium; Helaeomyia petrolei; Hydrothermal vent; Methanopyrus; Movile Cave; Radiotrophic fungus; Rio Tinto; Taq polymerase; Thermostability; Thermotogae … Read Article

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Taq DNA Polymerase
Master Mix Components. 0.1 unit/μl of Taq DNA polymerase, Reaction Buffer (pH 9.0), 400 μM •• Can be cycled up to 98°C to amplify challenging GC-rich templates others cannot (Figure 1). … Retrieve Content

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CLONTECH
Advantage-GC 2 combines the ability to amplify GC-rich sequences (up to 90% GC) with the established benefits of Advantage PCR: high efficiency and specificity due to the use of polymerase mixes and “hot-start” antibodies. The enzyme mix contains TITANIUM™ Taq DNA Polymerase—a … Doc Retrieval