Taq Polymerase Fermentas

TA Cloning – Wikipedia, The Free Encyclopedia
It is best if the PCR primers have guanines at the 5' end as this maximizes probability of Taq DNA polymerase adding the terminal adenosine overhang. Thermostable polymerases containing extensive 3´ to 5´ exonuclease activity should not be used as they do not leave the 3´ adenine-overhangs. … Read Article

Epidemiologic And Virologic Investigation Of Hand, Foot, And …
DNTP, 2.5 U Taq DNA polymerase (Fermentas), and Taq polymerase buffer (Fermentas). PCR cycling conditions included an initial denaturation step at 94°C for 5 min, fol- … View Full Source

Investigating Paraoxonase-1 Gene Q192R And L55M Polymorphism …
, 200 μM dNTPs, 20 pmol of each primer (Biobasic), and 1 U Taq polymerase (Fermentas, Inc.) in a thermocycler under the following conditions: 94°C … Fetch Document

Comparative Molecular Analysis Of Old Olive ( Olea Europaea L …
K0171), 1 unit of Taq DNA polymerase (Fermentas EP0402), MgCl 2, 30 ng of the primer and 20 ng of sample DNA. The mixtures were assembled at 0°C and then, transferred to thermal cycle, precooled … Fetch Full Source

Development Of A Quick Quantitative Real-time PCR For The In …
Green/ROX qPCR Master Mix (using Maxima Hot Start Taq DNA polymerase and a final MgCl 2 concentration: 2.5 mM) (Fermentas), included the following steps: 10 min at 95°C and 40 … Fetch Document

Taq Polimerasa – Viquipèdia
Aquesta activitat 5'-3' exonucleasa en la Taq, ha estat explotada per la PCR quantitativa amb sondes TaqMan o TaqMan real-time PCR, sofisticació de comercials de Taq ADN Polimerases, incloent Invitrogen, Applied Biosystems, Sigma, Promega, Roche Diagnostics, Qiagen, AttendBio, Bioneer i Fermentas. … Read Article

Pictures of Taq Polymerase Fermentas

Centre Of Excellence In Molecular Biology, University Of The …
32 Taq Polymerase 500unit/Vial Fermentas 10 Vial 9917 11644 14452 12531 14010 Aneela Y Maida Aslam Dr. Zia Maria Tayyab Sana Gull 33 Vancomycin Discs (MA-0102F50) Oxoid 3 Pk 11644 Maida Aslam … Access This Document

Product Information Last Updated: September 27, 2010 EvaGreen
DNA Polymerase . Manufacturer AmpliTaq Applied Biosystems Hot Start Taq . Fermentas Platinum Taq Invitrogen AccuPrime Taq with high fidelity . Invitrogen Platinum Tfi Invitrogen … Doc Retrieval

Assessment Of Biodiversity Based On Morphological …
Annealing and extension temperatures, Taq DNA polymerase concentration and MgCl 2 concentration in PCR. The final volume of the PCR reaction mixture was 25 µl containing 15 ng/ul DNA, lU/ul Taq polymerase (FERMENTAS INC USA), 2.5 mM … Read Full Source

Pictures of Taq Polymerase Fermentas

Determination Of ApaI And TaqI Polymorphisms Of VDR Gene In A …
Canada), 40 ng of DNA, and 0.3 unit of Taq DNA Polymerase (Fermentas). The touchdown PCR program was performed at 94˚C for 3 min (initial denaturation), 10 cycles, with 94˚C for 30s, 72- … Document Retrieval

Materials And Methods
DNA polymerase-I Roche Klenow polymerase Roche Lyticase Sigma Pepsin Sigma Proteinase K Sigma Restriction endonucleases Fermentas, NEB RNase A Sigma Taq DNA polymerase Fermentas … View Document

T7 RNA polymerase – Wikipedia, The Free Encyclopedia
T7 RNA Polymerase is an RNA polymerase from the T7 bacteriophage that catalyzes the formation of RNA in the 5'→ 3' direction. T7 polymerase is extremely promoter -specific and transcribes only DNA downstream of a T7 promoter. The T7 polymerase also requires a DNA template and Mg 2+ ion as … Read Article

Materials And Methods
MgCl2, 10% dimethyl sulfoxide (DMSO, Sigma), 1 U of Taq DNA polymerase (Fermentas) and 40 ng of template DNA. The PCR reaction was carried out in a PCR programmed ther- … View Document