Taq Polymerase Extension Rate

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PCR Brochure, March 2002
Thermostable DNA polymerase, exhibiting higher accuracy, elongation rate and processivity than any other commercially available DNA poly- •Greater yield – extension speed is 2X faster than Taq DNA polymerase and 5X faster than Pfu DNA polymerase (1) … Access Document

Хэрэглэгч:Bilguun.alt/Ноорог …
Can be utilized for PCR-mediated DNA mutagenesis, as higher Mn 2+ concentration increases the error rate Extension/elongation step: The temperature at this step depends on the DNA polymerase used; Taq polymerase has its optimum activity temperature at 75-80°C, and commonly a temperature of 72°C is … Read Article

Factors For Successful PCR – Polymerase Chain Reaction – PCR …
The traditional Taq polymerase has been replaced in many labs by higher fidelity enzymes (those that make Increased yield can be achieved by increasing the extension time about every 20 cycles, to Factors that Affect the Chemical Reaction Rate – Reaction Kinetics … Read Article

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Certificate Of Analysis Flexi DNA Polymerase: GoTaq Part …
The blue dye migrates at the same rate as a 3–5kb DNA fragment in a 1% agarose gel. Please be aware that excessive amounts of enzyme and excessively long extension times increase the likelihood of generating artifacts due to the intrinsic 5´→3´ exonuclease activity of Taq DNA polymerase. … Access Doc

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Taq PCR Handbook
Taq DNA Polymerase, the Taq PCR Core Kit, and the Taq PCR Master Mix Kit are devel oped, Extension rate: 2–4 kb/min at 72°C Half-life: 10 min at 97°C; 60 min at 94°C … Return Doc

Taq Polymerase Extension Rate

Applications
Eppendorf Taq DNA polymerase with its unique Mg2+ self-adjusting buffer (Taq Buffer advanced) and five reaction is too low, the polymerase extension rate is impaired – resulting … Return Document

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PCR REAGENTS FROM NEB
Extension Rate 1 kb/min 1 kb/min 2.4 kb/min 1 kb/min 1.2 kb/min 1 kb/min 1 kb/min 1 kb/min 0.5 kb/min 1 kb/min rate 5-fold lower than Taq DNA Polymerase, a characteristic derived in part from an … Read Here

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Biology 3950/7931 April 25, 2012 POLYMERASE CHAIN REACTION
Extension from the oligonucleotide primers Carried out near the optimal temperature of DNA synthesis catalyzed by the thermostable polymerase  72-78oC for Taq The polymerization rate of Taq is ~ 2000 nucleotides/min, but extension is usually a … Doc Viewer

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Manual: PfuTurbo Hotstart DNA Polymerase
PfuTurbo DNA polymerase has an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other Artifactual smears Decrease the amount of PfuTurbo hotstart DNA polymerase Reduce the extension time utilized … Retrieve Here

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Manual: Pfu DNA Polymerase
Product obtained using Pfu DNA polymerase. For Taq DNA polymerase-based PCR amplifications, an extension time of 0.5-1.0 minute/kb of the error rate of Pfu DNA polymerase can be avoided by maintaining the Mg2+ concentration above 1.5 mM, the total dNTP … Retrieve Document

واکنش زنجیره پلیمراز – ویکی‌پدیا
As higher Mn 2+ concentration increases the error rate during DNA Extension/elongation step: The temperature at this step depends on the DNA polymerase used; Taq polymerase has its optimum activity temperature at 75–80 °C, The Taq polymerase enzyme was also covered by patents. … Read Article

Taq Polymerase Extension Rate Images

Copies Should Be Used To Ensure Satisfactory Yields And And …
2-5 kb 20 sec/kb 2.5 sec/kb 10 sec/kb Extension time per cycle = extension rate (sec/kb) x amplicon length in kbBy simply reducing the extension time in each PCR cycle, a reduction of 20-70% in the total reaction time is achievable by usin KAPA2G Fast DNA Polymerase instead of wild-type Taq (see … Access This Document

Taq Polymerase Extension Rate

Enzymes
Top DNA Polymerase Top DNA polymerase is a novel thermostable polymerase that is more processive than Taq. In fact, the extension rate of Top is … Retrieve Doc