Taq Polymerase Elongation Rate

Pfu DNA Polymerase
Product Description: “Pfu DNA Polymerase” is a recombinant, thermostable, modified DNA polymerase from Pyrococcus furiosus. This enzyme is recombinant Pfu according to the published gene sequence. The reaction temperature of this enzyme is 68°C. The elongation rate is ~1/2 to Taq DNA polymerase … Return Doc

Ultrafast DNA Amplification With A Rapid PCR System
Duction of heat stable DNA polymerases such as the Taq DNA polymerase [3] and the development of the thermal slow rate is a result of the large thermal mass of the metal blocks in which denaturation,annealing and elongation take place.In … Fetch Content

Хэрэглэгч:Bilguun.alt/Ноорог …
Can be utilized for PCR-mediated DNA mutagenesis, as higher Mn 2+ concentration increases the error rate Extension/elongation step: The temperature at this step depends on the DNA polymerase used; Taq polymerase has its optimum activity temperature at 75-80°C, and commonly a temperature of 72°C … Read Article

Promoter (genetics) – Wikipedia, The Free Encyclopedia
Its presence allows a very high transcription rate. are recognized only by the sigma-70 protein that interacts with the prokaryotic RNA polymerase. Elongation … Read Article

واکنش زنجیره پلیمراز – ویکی‌پدیا
As higher Mn 2+ concentration increases the error rate during DNA Extension/elongation step: The temperature at this step depends on the DNA polymerase used; Taq polymerase has its optimum activity temperature at 75–80 The Taq polymerase enzyme was also covered by patents. … Read Article

Accuprime Technology For High-specificity Pcr Systems …
Amplification of genetic sequences, mostly caused by elongation of non-specifically annealed primers. The most widely used DNA polymerase, Taq DNA polymerase, has been plagued with a high error rate of … Document Retrieval

Green Taq DNA Polymerase- GenScript
Used for long DNA fragment amplification (>5 kb), the PCR yield is increased with longer elongation time (the elongation time as above was three minutes) 2. The Green Taq DNA polymerase can be Always use equal concentrations of all four dNTPs to help reduce the polymerase error rate. … Document Viewer

Thermophylic Polymerases A Users Guide Traditional Enzymes …
Error rate [1], [2] Application Taq Polymerase ++ ++ 10-5 ■■ Standard PCR / optimized for minimal by-product formation 100 bp 1 min elongation time DNA Ladder 100 bp DNA Ladder Figure 4 Jena Bioscience Taq Pol is capable of incorporating up to 3 kb per min … Access Document

Taq DNA Polymerase DNA-free
Taq DNA Polymerase DNA-free From Thermus aquaticus BM, recombinant (E.coli) Deoxynucleoside-triphosphate: DNA Sequencing/ cycle sequencing Enzyme Properties Volume activity 5 U/μl Error rate* approx. 1.3×10 94°C 15-30 sec 25-40x Annealing # 45-65°C 30-60 sec Elongation + 72°C 45 sec – 3min Final elongation 72°C 10 … Get Doc

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Has 3 steps Polymerase (e.g. Taq polymerase from Thermus aquaticus Mg2+ dNTPs Template Primers What are the factors affecting: Annealing temperature Elongation time primer length, primer GC content, salt concentration enzyme processivity, fragment length (Taq polymerase has an extension rate … Retrieve Doc

Biology 3950/7931 April 25, 2012 POLYMERASE CHAIN REACTION
polymerase  72-78oC for Taq The polymerization rate of Taq is ~ 2000 nucleotides/min, but extension is usually a Elongation: 72oC for 1 min  35 cycles of denaturation, annealing and elongation, heated lid at 104oC … Get Document

The rate polymerization of Taq polymerase is 1000 bp/min. Your supervisor has asked you to PCR amplify an interesting DNA sequence that is of normal GC content and 1.5 kb in length. What elongation/polymerization time would you use in your PCR program? … Access Doc

Pfu-X Polymerase
Increased fidelity of DNA synthesis compared to Taq polymerase. higher fidelity compared to standard Pfu polymerase. ER Pfu-X Polymerase = 0.25×10 -6 The error rate (ER) of a PCR reaction is 68°C 30 sec/kb Final elongation 68°C 30 sec/kb 1x … Get Content Here