Taq Polymerase Cetus

Taq Polymerase Cetus

DETECTION OF BCL-2 GENE REARRANGEMENT IN FOLLICULAR LYMPHOMA …
Each dNTP; 20 pmol/L of each primer; 1 U Taq polymerase (Ampli-Taq, Perkin Elmer-Cetus, Norwalk, CT, USA) and 1 µL of DNA digest in a final volume of 25 … Fetch Full Source

Polymerase-Kettenreaktion – Wikipedia
Mullis arbeitete zu dieser Zeit für die kalifornische Biotech-Firma Cetus und wurde mit einer Prämie von 10.000 US-Dollar abgefunden. Jahre später verkaufte Cetus dann die Rechte an der PCR-Methode mitsamt dem Patent für die von ihm verwendete DNA-Polymerase Taq an die Firma Roche für 300 … Read Article

Taq Polymerase Cetus Photos

Genomic Fingerprinting By Arbitrarily Primed Polymerase Chain …
Tions of 0.2 mM, 1 U of Taq polymerase (Perkin Elmer- Cetus), and 5 FCi of [cY-~~PI~CTP was added, and the preparation was subjected to 30 additional high-stringency … Retrieve Content

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Biotechnology Product Development: Taq polymerase
Strain YT-1 (ATCC # 25104) is source of Taq used in Cetus patents. According to Brock, several isolates were screened for activity by Cetus but YT-1 Clone Taq polymerase gene into vector pET-17b. Find sequence in Genebank. Design primers (Primer 3, manual) … Access This Document

Taq Polymerase Cetus

Prevalence Of Canine Coronavirus And Parvovirus Infections In …
Thermocycler (Perkin Elmer Cetus, Norwalk, USA). Briefly, 10 µl of the respective DNA sample were added to a reaction mixture containing 1.5 units Taq polymerase (Perkin Elmer Cetus), 250 µM … View Document

Modern Evolution Of Genetics Timeline – Wikipedia, The Free …
1983 – Kary Mullis of the Cetus Corporation drafted a technique for amplifying DNA through a cloning procedure that Heat applied to the DNA segment causes it to separate, allowing the DNA polymerase to bind with the single strand of DNA. Taq polymerase (heat activated polymerase that synthesizes … Read Article

Images of Taq Polymerase Cetus

Use Of Polymerase Chain Amplification Reaction For The …
Primer, 200 //M each dideoxynucleotide, 2.5 U Taq polymerase, 10 mM Tris HC1, pH 9.3, 50 mM KC1, 2.5 mM MgCl2, and 0.01% gelatin. Nucleotides were supplied by Perkin-Elmer-Cetus. … Fetch Doc

Images of Taq Polymerase Cetus

Optimization BASIC CONSIDERATIONS And Troubleshooting In PCR
Taq polymerase readily incorporates uracil in place of thymine into PCR from GIBCO-BRL and Perkin-Elmer Cetus. One drawback to these procedures … Access This Document

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Tissue- And Development-specific Alternative RNA Splicing …
Tion of 0.2 mM, and 5 units of Taq. polymerase (Cetus). Thirty cycles of amplification were performed using an automated Perkin-Elmer … Document Viewer

Taq Polymerase Cetus Photos

PCR-Single-Strand RapidIdentification OfBacteria By …
Digest); right lanes, Super-Taq polymerase. (A) Reactions without added DNAtemplate.-, no DNasetreatment; PH, DNase I (Phar- Cetus, Norwalk, Conn.). After amplification, 5 pl of the amplified productwasrun ona 1%agarose gel in 0.5x TBE. … Fetch This Document

Images of Taq Polymerase Cetus

Capacity Of Nine Thermostable DNA Polymerases To Mediate DNA …
1.25 U of rTth DNA polymerase (Perkin-Elmer Cetus); the PCR buffer of Taq DNA polymerase (Thermus aquaticus) contained 10 mM Tris-HCl, 1.5 mM MgCl … Fetch Doc

Taq Polymerase Cetus Pictures

David R. Engelke, *J Alexandra Krikos,? Mary E. Bruck,t$ And …
Mercial Tuq DNA polymerase (Cetus Corp.). Enzyme activity given in Table 1 is expressed as previously de- Although the Taq DNA polymerase produced by these methods has performed well in a wide variety of … View This Document