Taq Polymerase Blunting

Nucleotide primer, and 0.5 unit of Taq DNA polymerase (Perkin- Elmer Cetus). blunting, the purified cDNA fragments were ligated with SmuI- digested pUC18 and introduced into Escherichia coli JM109 by trans- … View This Document

Cloning Of Human Erythropoietin Gene In PSinCMV Vector
blunting of staggered ends generated by EcoRI enzyme, T4 DNA polymerase was used. each of gene specific forward primer and BGH as reverse primer and 3 units of Taq DNA polymerase in 1x PCR buffer. … Get Content Here

Crosslinking Of Cells, Fragmentation And Immnuoprecipitation …
Blunting, Ligation of Linker to DNA, and Amplification by PCR The immunoprecipitated DNA is usually in so small a quantity (1-10 ng of total) that enzyme mix [8 µl of dH2O, 1 µl of Taq DNA polymerase (5 units/µl), 1 µl of ThermalPol … Read Full Source

Triiodothyronine Utilizes Phosphatidylinositol 3-kinase …
Human genomic DNA with Taq polymerase and 5 0MCL1 5 -AATCCCGGGTATGTCTCTCAGCACCT- SmaI, blunting SacI end with Klenow, and re-ligation) and therefore, contained the K295 to C16 part of this … Read More

Fragment Length Polymorphism-coupled
One-half microliter ofresuspended restriction fragments was treated in 10-,lI blunting reaction mixtures[50 mM Tris-HCl, pH 8.0/6 mM MgCl2/50 mM then used to setup on ice 10-pd primer extension reaction mixtures under the above described conditions containing 50 jiM dNTPs, 1 unit of Taq DNA polymerase, … Read Full Source

1 Taq DNA Polymerase and Ultrapure dNTPs Web Tool Focus 8 Double Digest Finder Optimize your blunt-end ™cloning reactions with the Quick Blunting Kit from NEB. … View Doc

Supplements 1 17 2012
Blunting and 5’ phosphorylation Fragmented (for application 2 and 3 also size selected) and concentrated DNA is blunted and 5’ Danielsson, Fennell, Russ, Jaffe, Nusbaum and Gnirke 2011), AccuPrime HF Taq DNA polymerase with … Fetch Here

Differential Activation Of The Connexin 43 Promoter By Dimers …
Boring mouse myometrium using Taq polymerase (MBI Fermentas, Flamborough, Ontario, Canada) and the following primers: JunB ApaI, blunting, and then digesting with XhoI. The c-Fos coding region was then ligated into the pcDNA3.1 vector at XhoI/ApaI(blunt). … Return Doc

Endosymbionts Of Ticks And Their Relationship To Wolbachia …
Triphosphate, 20 pmol or 10 mM each primer, 2.5 U of Taq polymerase (Perkin with a DNA blunting kit (TAKARA) and ligated into pBluescript II (Strat … Document Retrieval

High Prevalence Of Inducible Erythromycin Resistance Among …
And1UofTaq polymerase. PCR products were resolved by electrophoresis on 1.5% agarose gels. an inducible phenotype (iMLS isolates) with typical blunting of the clindamycin zone of inhibition, and 17 (45%) exhibited the … Document Retrieval