Taq Polymerase Amplicon Length

Copies Should Be Used To Ensure Satisfactory Yields And And …
2-5 kb 20 sec/kb 2.5 sec/kb 10 sec/kb Extension time per cycle = extension rate (sec/kb) x amplicon length in kbBy simply reducing the extension time in each PCR cycle, a reduction of 20-70% in the total reaction time is achievable by usin KAPA2G Fast DNA Polymerase instead of wild-type Taq (see … Access Content

GoTaq Long PCR Master Mix Technical Manual #TM359
Recombinant Taq DNA polymerase is bound by proprietary antibodies that inhibit activity at lower temperatures. Amplicon Length:GoTaq® Long PCR Master Mix is designed for PCR targets greater than 5kb. … Fetch Here

Advantage Genomic LA Polymerase Mix Protocol-at-a-Glance
1 kb of amplicon. TABLe I. COMPONeNTS OF PCR ReACTIONS Reagents Volume/Final Concentration per Reaction Advantage Genomic LA Polymerase Mix (Cat. No. 639152) is composed of a full-length Taq DNA polymerase, a small amount of proofreading enzyme, and a hot-start … Retrieve Document

Enzyme Blend Q
A 742-bp fragment of DNA from these samples was compared to that of Taq DNA polymerase (Figure 1A). Restorase™ increased amplicon yield over Taq when increased amplicon length comes increased occurrences of polymerase stalling due to … View This Document

PCR Bias In Ecological Analysis: A Case Study For …
On the basis of the length and restriction polymorphism of the amplicons. quantification techniques in which Taq polymerase activity or the accumulation of the amplicon is directly monitored during … Retrieve Doc

High-Fidelity DNA Polymerase Thermo Scientific Phusion Use 15 …
On amplicon length and difficulty. It is not recommended to exceed 2 U/50 µl (0.04 U/µl), especially for amplicons The optimal annealing temperature for Phusion DNA Polymerase may differ significantly from that of -based polymerases. Taq … View Doc

SNP Genotyping – Wikipedia, The Free Encyclopedia
Taq DNA polymerase’s 5’-nuclease activity is used in the TaqMan assay for SNP genotyping. Either you butt up the primers right up to the SNP in question (small amplicon genotyping, Liew, 2004) or amplify a larger region (100-400bp in length) for scanning purposes. … Read Article

Polymerase Chain Reaction – Wikipedia, The Free Encyclopedia
Annealing temperatures for each of the primer sets must be optimized to work correctly within a single reaction, and amplicon sizes. That is, their base pair length The Taq polymerase enzyme was also covered by patents. There have been several high-profile lawsuits related to the technique … Read Article

MINIREVIEW Polymerase ChainReaction: Trenches Benches
Stranded products, two are of a length defined t product fied DNAthat prevent Taq polymerase from traversing the ion mixtures, solution for an existing (T-containing) amplicon contamina- … Fetch Document

Product Length, Dye Choice, And Detection Chemistry In The …
R-primer; 0.06X/µL Titanium Taq Polymerase (Clonetech). The beads were washed and analyzed by allele-specific exten- we failed to observe an increase in amplicon length (see Figure S2 in the Supporting Information). … Access Content

Molecular And Immunological Methods
This depends on the dye used, the sequence amplified, the length of the amplicon, the optical properties of the platform, data acquisition and instrument * * Taq polymerase activities: Activity optimum at 75-80 ºC 5’-3’ DNA polymerase (~100 bases/second) No 3’-5’ exonuclease activity (ie no … Read Here

One-Step RT-PCR For Long Templates SuperScript
The Taq DNA polymerase in the blend is complexed with a proprietary antibody that inhibits enzyme time varies with the size of the amplicon (approximately 1 min per 1 kb of amplicon).  For all targets up to 3 kb, 1 μL of RT/Platinum® Taq HiFi Mix is sufficient.  As the length of the … View Document

Amplicon – Wikipedia, The Free Encyclopedia
An amplicon is a piece of DNA formed as the product of natural or artificial amplification events. For example, it can be formed via polymerase chain reactions (PCR) or ligase chain reactions (LCR), as well as by natural gene duplication. Artificial amplification can be used to determine sex … Read Article