Taq Polymerase Alternatives

METHODOLOGY Open Access Development Of A Fast PCR Protocol …
Methods: Fast PCR conditions were achieved by substituting the traditional Taq polymerase for SpeedSTAR™ HS ging alternatives to traditional approaches. Some proto-cols are already being offered to investigators for … Retrieve Doc

Biotechnology Terms – Biotechnology Research – Science Research
Research on the use of stem cells from non-embryonic sources has received more attention in recent years as scientists in some countries, particularly the US, are forced to search for publicly accepted, ethical alternatives. … Read Article

Northern Blot Analysis With Digoxigenin In PCR-labeled Probes …
Acid blotting as alternatives to techniques employing radioactivity [1]. The aim of this study is to develop a cDNA and then amplified by Taq Polymerase (Roche Molecular Biochemicals). Reverse transcription was … Read Content

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PCR Polymerase Chain Reaction Agarose Gel Electrophoresis Separating and visualizing DNA fingerprints PCR is based upon DNA Replication PCR technique PCR technique Process involves alternatives cycles of heating & cooling this mixture. Melting DNA double helix Anneal primers Taq polymerase copies … View Doc

Microchip-Based Purification Of DNA From Biological Samples
We recently examined several low-cost alternatives for increasing the silica surface area inside a microchannel. Taq polymerase, primers, and other PCR reagents for amplification of the 500-bp ì-DNA fragment were … Get Doc

Polymerase Chain Reaction Detection Of Trypanosoma Cruzi In …
And robust alternatives to immunologic diagnosis that prom ise both high specificity and high sensitivity. sample DNA, 1 U Platinum Taq DNA polymerase (Invitrogen, Carlsbad, CA), and other components as specified by the … Fetch This Document

Generating A Synthetic Genome By Whole Genome Assembly …
The Institute for Biological Energy Alternatives 1901 Research Blvd., #600 K1914-1) and either 0.2, 0.5, or 1µl of the Taq ligation product. The polymerase mixture … Return Doc

In Situ PCR: A Review
Higher temperatures and longer times may lead to the loss of enzyme activity since the half-life of Taq polymerase is approximately 5 minutes at 97o C Several alternatives to reduce the artifact have been attempted with only partial success. … Fetch Document

Pfu DNA polymerase – Wikipedia, The Free Encyclopedia
Pfu DNA polymerase is hence superior to Taq DNA polymerase for techniques that require high-fidelity DNA synthesis, but can also be used in conjunction with Taq polymerase to obtain the fidelity of Pfu with the speed of Taq polymerase activity. … Read Article

York 10021 PCR Has Facilitated The Development Of A Variety …
Complement, or as alternatives to, PCR. (6'7) From its initial detailed reports in ( 9 Taq polymerase 3' to each of the two PCR primers. After PCR amplification, Taq polymerase is inactivated by 97~ for 25 … Retrieve Content

Mode Of Action And Application Of Scorpion Primers To …
Which can be used as alternatives, the Scorpion system does not require a separate probe. either GoldStar Taq polymerase or 5′–3′ exonuclease deficient Taq polymerase. … View Full Source

PCR Assisted Biochemistry
TAQ polymerase optimum at 72° C •Alternatives: Thermococcus litoralis, Pyrococcus furiosus Thermocycler … Fetch Full Source

Polymerase Chain Reaction – Wikipedia, The Free Encyclopedia
A related patent battle over the Taq polymerase enzyme is still ongoing in several jurisdictions around the world between Roche and Promega. The legal arguments have extended beyond the lives of the original PCR and Taq polymerase patents, which expired on March 28, 2005. … Read Article