Taq Polymerase Add

Polymerase Chain Reaction – PCR – How Pcr Works
Under controlled conditions, small segments of DNA are generated by enzymes known as DNA polymerases, that add complimentary deoxynucleotides (dNTPs) the sample is reheated to 72 degrees, the ideal temperature for Taq DNA Polymerase, for elongation. … Read Article

Wikipedia:Reference Desk/Archives/Science/2008 May 9 …
Sensitivity of Taq polymerase to heat . My lab supervisor I add master mix, template, then lastly the enzyme. However, that being said, if you are getting "contaminated" looking lanes, it's most likely the primers and the annealing temperature. … Read Article

GEFEK – Wikipedia, The Free Encyclopedia
GEFEK, the Association for the Promotion of Independent Disease Control in Developing Countries ("G esellschaft zur F örderung E igenständiger K rankheitsbekämpfung in Entwicklungsländern") was created by scientists of the University of Giessen, Germany, in 2004 and is officially registered … Read Article

Wikipedia:Reference Desk/Archives/Science/2008 March 2 …
If Taq polymerase is good to go at 95 C, why does it need to be stored at -20? How quickly will it break down at room temperature? Then I add template to the reaction tubes and then I add Taq to the master mix and distribute it among the reaction tubes. … Read Article