Taq Polymerase 5′ Exonuclease Activity

Taq Red DNA Polymerase Kit Components
polymerase and a 5'3' exonuclease activity. The enzyme lacks a 3'5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A overhang, which makes the enzyme ideal for 4/23/2012 11:39:41 AM … Retrieve Content

HE Printed In U.S.A. Biochemical Analysis Of Point Mutations …
Sequence, the 2.4-Å resolution x-ray structure of the 59-39 exonucleolytic domain of Taq pol (9), and 2.5-Å resolution x did not affect the 59-39 exonuclease specific activity. However, this fusion resulted in a 5-fold reduction of the polymerase … Retrieve Doc

Technical Tipslill|ll
Rococcus furiosus/s~ In contrast with Taq DNA polymerase, Pfu DNA polymerase possesses a 3'—~ 5' exonuclease (proof- reading) activity that is responsible for White), pp. 39-45. Academic Press, San Diego. 2. Knoth, K., S. Roberds, C. Poteet … Document Retrieval

COA: Taq DA Polymerase, #EP0406
Taq DNA Polymerase catalyzes 5'→3' synthesis of DNA. The enzyme has no detectable 3'→5' proofreading exonuclease activity and possesses low 5'→3' exonuclease activity. et al., Producing single-stranded DNA probes with the Taq DNA polymerase: a high yield protocol, BioTechniques, 10, 35-39 … Retrieve Doc

Taq PCR Handbook
Appendix I: Control of Contamination 39 Ordering Information 40 Exonuclease activity assay: Linearized DNA is incubated with Taq DNA Polymerase in QIAGEN PCR Buffer. Exonuclease activity per unit enzyme is indicated under “Exo”. … Access Full Source

Taq PCR Handbook
Switzerland QIAGEN AG Auf dem Wolf 39 • 4052 Basel 5'–3' exonuclease activity: Yes Extra A addition: Yes Exonuclease activity assay: Linearized DNA is incubated with Taq DNA Polymerase in QIAGEN PCR Buffer. Exonuclease activity per unit … Access This Document

Vol. 276, No. 7, Issue Of February 16, Pp. 5044–5051, 2001 …
Bases in vivo would facilitate 3 9-5 exonuclease removal of the nascent nucleotide. Exonucleolytic (39-59) proofreading activity detectable endogenous polymerase and nuclease activities. Each of the Taq pol Is that retain at least 10% activity relative to WT enzyme at … Doc Retrieval

T-Taq: Transgenomic Taq DNA Polymerase
The enzyme possesses 5’ 3’ DNA polymerase activity and 5’ 3’ DNA exonuclease activity, but not 3' 5’ DNA exonuclease activity. T-Taq is >95% homogeneous by 10/30/2008 9:39:21 AM … Access This Document

HotStarTaq PlusPCR Handbook
Appendix D: Challenging PCR Assays 39 Appendix E: RT 5'–3' exonuclease activity: Yes Extra A addition: Yes 94 kDa Taq DNA Polymerase from QIAGEN. HotStarTaq Plus DNA Polymerase is provided in an inactive state with no polymerase activity at ambient temperatures. … Read More

Wikipedia:Graphic Lab/Images To Improve/Archive/Apr 2008 …
68.39.174.238 15:15, 5 March 2008 (UTC) PS. New York's appears to be the flag, but without the crown: Seal of New York. what the author was trying to convey was that the quenched probe is added to the target and then cleaved by the exonuclease activity of Taq DNA polymerase. … Read Article

Taq DNA Polymerase
Thermostable recombinant DNA polymerase encoded by a modified gene from Thermus aquatic us species and expressed in E.coli. Its recombinant nature ensures utmost purity, reproducibility and processivity. Top Taq processes an initial 5" → 3' exonuclease activity and 1/22/2011 8:39:03 AM … Retrieve Here