Takara Taq Polymerase Protocol

KAPA Taq™ EXtra HotStart ReadyMix With Dye
- For standard PCR, replace Taq with no protocol change. – Products can be cloned in T-overhang cloning vectors. Technical Data Sheet 3. Background Taq DNA polymerase lacks Taq polymerase does not perform well due to amplicon length, or for purposes other than research, please contact Takara … Access Content

RAPD 使用キット GIBCO BRL Taq DNA polymerase
RAPD 使用キット GIBCO BRL Taq DNA polymerase TaKaRa dNTP mix 反応液の調整(1サンプルあたり) H2O 13.9μl 10×バッファー 2μl primer (20μg/ml) 0 … Visit Document

Product Code Kit Size KAPA LongRange HotStart DNA Polymerase
The KAPALongRange HotStart PCR system is a blend of Taq DNA polymerase and a modified archaeal (Type B) DNA modification of the normal Taq protocol. However, as the reaction becomes more difficult (longer to use this product for purposes other than research, please contact Takara Bio Inc., Seta 3 … Get Content Here

Taq polymerase – Wikipedia, The Free Encyclopedia
Taq polymerase is a thermostable DNA polymerase named after the thermophilic bacterium Thermus aquaticus from which it was originally isolated by Thomas D. Brock in 1965. … Read Article

One Step PrimeScript RT-PCR Kit (Perfect Real Time)
Protocol: Note: This product combines the high performance of TaKaRa Ex Taq TM HS, which is an enzyme for hot start PCR utilizing Taq antibody. No need to heat at 95℃ for (5-) 5 min. as the initial denaturation, required for chemically modified Taq polymerase. … Access Document

Product Information Last Updated: September 27, 2010 EvaGreen
Applied Biosystems Taq polymerase New England Biolabs ExTaq . Takara Taq DNA polymerase . Qiagen For chemically-modified enzyme, such as AmpliTaq Gold, EvaGreen TM qPCR Basic Mix still produces good but not the best results. … Doc Retrieval

FAQs – SYBR Premix Ex Taq
Takara has used cDNAs which corresponded to 1 pg, 10 pg, 100 pg, 1 ng, 10 the protocol for the LightCycler on page 9 of our product manual for information regarding Is SYBR® Premix Ex Taq™compatible with an RT polymerase? … Read Full Source

ENDEXT Technology
SP6 Polymerase (80 U/µl) 0.6 2.4 U/µl 0.25 1 U/µl RNase Inhibitor (80 U/µl) 0.3 1.2 U/µl 0.25 1 U/µl Nuclease-free water 6.1 – 11.5 – Total 20 20 • The following protocol uses TaKaRa Ex Taq™ and a related buffer from TaKaRa Bio … Read Document

Gene Cloning – Molecular Genetics Techniques – Genetic …
Polymerase Chain Reaction. The discovery of thermostable DNA polymerases, such as Taq Polymerase, made it possible to manipulate DNA replication in the laboratory and was essential to the development of PCR. … Read Article

Long PCR
Determine optimal magnesium concentration Enzyme Mismatch occurs during synthesis, Taq DNA polymerase References A new protocol for highly efficient amplification of long PCR products (Susan Kobsch Enzyme amount: too high, non-specific reactions 2.5 units of TaKaRa LA Taq /50 ml * … Doc Retrieval

Polymerase Chain Reaction Optimization – Wikipedia, The Free …
Magnesium is required as a co-factor for thermostable DNA polymerase. Taq polymerase is a magnesium-dependent enzyme and determining the optimum concentration to use is critical to the success of the PCR reaction. Some of the components of the reaction mixture such as template concentration, dNTPs … Read Article

FideliTaq ™ PCR Master Mix (2X) Product Number 71182 100 …
By TaKaRa Bio Inc. HotStart-IT Taq DNA Polymerase—Patent pending. PrepEase products are covered under European Patent EP 0496822 and US Patent 6,428,703. This standard protocol applies to a single reaction where only template, primers … View Full Source

Genomic Signature Tags (GST) Protocol Required Reagents And …
Genomic Signature Tags (GST) Protocol Required reagents and recommended suppliers T4 DNA ligase 350U/µl from Takara Biotechnology equals ~2.8 Weiss U/µl Platinum Taq DNA Polymerase High Fidelity (Invitrogen) Preparation of linker cassettes … View Full Source