Takara Hot Start Taq Polymerase

The PCR mixture was prepared using OmniMix HS beads (Fisher Scientific, Pittsburgh, PA) reconstituted according to the manufacturer s instructions, which contained 1.5 U TaKaRa hot-start Taq polymerase, 200 m M deoxynucleoside triphosphates, 4 mM MgCl 2 , 25 mM HEPES, pH 8.0 per 25m l of reaction … Return Document

Takara Hot Start Taq Polymerase (Takara Bio Inc., Japan), 2.5 ml Takara 103 PCR buffer (Takara Bio Inc., Seta 3-4-1, Otsu, Shiga 520-2193, Japan), 200 mM dNTP mixture (Takara … Fetch Document

KAPA LongRange HotStart ReadyMix With Dye
The KAPA LongRange PCR system is a blend of Taq DNA polymerase and a modified archaeal (B-family) DNA polymerase. Takara Bio reserves all other rights, and this product may not be used in any manner other than as provided herein. … Retrieve Here

Comparison Data For Taq Master Mix (1)
Taq DNA Polymerase. α-Taq DNA Polymerase Pfu DNA Polymerase : Hot Start Activation 5X Green Reaction Buffer 5X Colorless Reaction maker GeneAll ABI TaKaRa product name α-Taq. GeneAmp High Fidelity … Fetch This Document

Product Information – Thermo Scientific Phire Hot Start II …
Hot Start II DNA Polymerase provides 2-fold fidelity compared to Taq DNA polymerase. The hot start modification of the polymerase is based patents owned by Takara Bio, Inc.. This product is also licensed under U.S. Patents … Fetch Here

RT-PCR stands for “Reverse Transcriptase – Polymerase Chain Reaction”. In this procedure, we use Reverse transcriptase to make cDNA, and TaKaRa Ex Taq™, Hot-Start Version, Premix (Takara TAK RR030A) Primers for the specific region of the genome you wish to amplify … Fetch This Document

Polymerase Chain Reaction Optimization – Wikipedia, The Free …
Taq polymerase is a magnesium-dependent enzyme and determining the optimum concentration to use is critical to the success of the PCR reaction. Non-specific priming during reaction preparation at lower temperatures can be prevented by using "hot-start" polymerase enzymes whose active site is … Read Article

Pfu DNA polymerase – Wikipedia, The Free Encyclopedia
Pfu DNA polymerase is hence superior to Taq DNA polymerase for techniques that require high-fidelity DNA synthesis, but can also be used in conjunction with Taq polymerase to obtain the fidelity of Pfu with the speed of Taq polymerase activity. … Read Article

USB HotStart-IT FideliTaq DNA Polymerase
Precious samples, like with chemically-modified Taq USB HotStart-IT FideliTaq DNA Polymerase combines a novel hot start method designed and developed at Affymetrix with FideliTaq DNA Polymerase technology is licensed under US Patent No. 5,436,149 owned by TaKaRa Bio Inc. Taq DNA Polymerase—sold … Retrieve Document

Comparison Of DNA polymerases For Real-time PCR With SYBR …
Performers were SpeedSTAR HS from Takara and KAPA2G Robust from Kapa Biosystems. Introduction DNA polymerase from Thermus aquaticus (Taq) was the fi rst to Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR. … Access Content

PCR Just Got Easy
A Takara Bio Company www.clontech.com United States/Canada: +1.800.662.2566 • Asia Pacifi c: +1.650.919.7300 Advantage Genomic LA is a premium enzyme blend containing full-length Taq polymerase, a small amount of proofreading enzyme, and a hot start antibody. … Document Viewer

United States Department Of Agriculture Agricultural …
Pyruvate (mBPWp) with ACV  Chromagenic agar plates (Example: DRG Chromagenic agar, etc.)  L-EMB Agar  MacConkey Agar  LST broth  PCR grade water, nuclease free  OmniMix® HS PCR master mix reagent beads, or equivalent, containing 3 units TaKaRa hot start Taq polymerase … Get Document