Surestart Taq Polymerase

BioMed Nutrition & Metabolism Open Access Research
I, SureStart Taq DNA polymerase (50 units/ml) with hot start capability, and 20 nM of fluorescein for experimental plate well factor collection on the Mx3000P™ Real-Time … Read Content

Development/Plasticity/Repair …
Chloride, 2.0 lof20mM dNTP mix, 0.1 lof5U/ l SureStart TaqDNA polymerase,2.9 lwater,and1.0 lcDNAfromtheRTreactionandrunfor 40 cycles at 95°C for 30 s and 72°C for 60 s in an ABI 9700 Thermal Cycler … Read Full Source

Extracellular Superoxide Dismutase Functions As A Major …
PCRs were performed using SureStart Taq Polymerase (Stratagene, La Jolla, CA) at 95 C for 5 min, and 34 cycles at 94 C for 1 min, 60 C for 1 min, 72 C for 1 min. … View Full Source

Mel-18, A Polycomb Group Protein, Regulates Cell …
Consisted of an initial activation of SureStart Taq DNA polymerase at 95°C for 10 min, followed by 40 cycles of 95°C for 30 s, 58°C for 1 min, and 72°C for 1 … Doc Viewer

Development Of A Assay For Detection
2.5mM, SureStart TM Taq DNA polymerase and standard NTPd mix), 0.5 µL of ROX reference dye (Invitrogen, Carlsbad, CA), 4 nM each of primers virD2A and virD2E. … Return Document

Real-Time PCR For Quantification Of
Rhodamine ([ROX]), and 2.5 U of SureStart Taq DNA polymerase. A volume of up to 5 l of DNA was added. The PCRs were performed in an Mx4000 multiplex quantitative PCR system (Stratagene). … Fetch Here

Intracellular Expression Profiles Measured By Real-time PCR …
CDNA, 1U SureStart Taq DNA polymerase (Stratagene-Europe), 2.5ml of reaction buffer (10 ), 3mM MgCl 2, 0.4mM dNTP mix, 50000-fold diluted SYBRGreen I … Visit Document

Multiplex Quantitative Real-Time Reverse Transcriptase PCR …
PCRs (50l) contained 100 nMofsubgroup II forward and reverse primers, 100 nMofsubgroup III forward and reverse primers, 100 nMofsubgroup IV forward primer, 200 nMofsubgroup IV reverse primer, 200 nMofeach molecular beacon, 4 mM deoxynucleoside triphosphate, and 5 Uof SureStart Taq DNA polymerase in 1 … Get Document

Evolutionary Dynamics Of GII.4 Noroviruses Over A 34-Year …
Dye, 1.25 U of SureStart Taq DNA polymerase, 1 l of reverse transcriptase, 800 nM each of primer MGBGI_F and MGBGI_R, 500 nM primer MGBGII_F, 300 … Read Full Source

Borchure – Quantitative PCR Systems
QPCR Master Mixes utilize SureStart Taq ® DNA polymeraseb for high specificity, and StrataScript Reverse Transcriptase® d owned by F. Hoffmann-La Roche Ltd, covering the Polymerase Chain Reaction (“PCR”) process, to practice the … Fetch Document