Sigma Aldrich Taq Polymerase

5 Materials And Methods
Spermine Sigma-Aldrich (Steinheim, Germany) Sucrose Merck (Darmstadt, Germany) T4-DNA ligase Fermentas (St. Leon-Roth, Germany) Taq polymerase Roche (Mannheim, Germany) … Read More

Affymetrix Chromatin Immunoprecipitation Assay Protocol
Taq polymerase Adapter 3' 5' 5' 3' U UUU U Proteins Antibody Protein-A beads Uracil DNA Glycosylase EDTA Disodium Salt, 0.5M solution (100 mL)* Sigma-Aldrich E7889 DMSO* Sigma-Aldrich D5879 … Doc Viewer

TaqMan – Wikipedia, The Free Encyclopedia
The TaqMan probe principle relies on the 5´–3´ exonuclease activity of Taq polymerase to cleave a dual-labeled probe during hybridization to the complementary target sequence and fluorophore-based detection. As in other real-time PCR methods, the resulting fluorescence signal permits quantitative … Read Article

Thermophilic Thermotoga Maritima Ribose-5-phosphate Isomerase …
All chemicals were regent grade, purchased from SigmaAldrich (St. Louis, MO, USA) and Fisher Scientific (Pittsburgh, PA, USA), un-less otherwise noted. Ribose-5-phosphate and ribulose-5-phos-phate were purchased from SigmaAldrich. Restriction enzymes, Taq DNA polymerase, T4 ligase, and a broad … Fetch This Document

PROTOCOLE PCR TAQ MAN
MGB Eclipse PCR Reagent Kit for SNPs provided by Sigma-Aldrich or from Nanogen. Mix for preparation of 2X MasterMix for one 384 wells-plate : MGB Eclipse Premix 940 µL. JumpStart Taq DNA Polymerase (2.5unit/µL) 60 µL … Access Content

TaqBead™ Hot Start Polymerase
*To prepare TaqBead™ Hot Start Polymerase, Taq DNA Polymerase is incorporated into wax beads. • nuclease-free light mineral oil (e.g., Sigma-Aldrich Cat.# M5904). Do not … View Document

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Taq DNA Polymerase 10X buffer with MgCl2 JumpStart REDTaq DNA Polymerase Company: Sigma-Aldrich Corporation Other titles: Sigma-Aldrich Checklist … View Doc

Master Mix Kits For TaqMan® Probes
Exonuclease activity of the DNA polymerase degrades the annealed probe and releases the fl uorophore into solution. 4352042), JumpStart™ Taq ReadyMix™ for Quantitative PCR (Sigma-Aldrich, Cat. No. D7440), Platinum® Quantitative PCR … Retrieve Full Source

Polymerase Chain Reaction – Wikipedia, The Free Encyclopedia
A related patent battle over the Taq polymerase enzyme is still ongoing in several jurisdictions around the world between Roche and Promega. The legal arguments have extended beyond the lives of the original PCR and Taq polymerase patents, which expired on March 28, 2005. … Read Article

Selection Of Aptamers By Systematic Evolution Of Ligands By …
Single-strandedDNA-bindingproteinfromE.coli,Taq DNA polymerase, fluorescein, and buffer components were obtained from SigmaAldrich (Oakville, Canada). … View Doc

Protocol Submitted With Peer-reviewed Article – SPLICE: A …
Reagents Source of Genomic DNA (e.g., liver) Tris Base (Cat# T1503-1KG; Sigma-Aldrich, Gillingham, UK) Ethylenediaminetetraacetic Acid (EDTA mM dNTP mix and 25 mM MgSO 4 (Cat# 71086-3; Novagen, Nottingham, 4 (Cat# 71086-3; Novagen, Nottingham, 4 UK) or Platinum Taq DNA Polymerase Kit, with … Content Retrieval

PCR PROTOCOL FOR CDNA ARRAYS ON MEMBRANES
 20%/0.025mg/ml Sucrose/Cresol (Cresol, Sigma-Aldrich, cat# 114480)  MJ Research Tetrad PCR machine (MJ Research, cat# PTC-225) Thaw all reagents on ice, leaving Taq Polymerase @ -20 °C until needed. … Retrieve Document