Roche Taq Polymerase Error Rate

HelpCorner-Amplification Of Nucleic Acids
FAQ: What’s the statistical error rate of FastStart High Fidelity PCR System? HelpCorner: Please note that defining error rates of a DNA Polymerase makes sense only when it is compared directly with Taq DNA Polymerase. Therefore, Roche Applied Science defines the error rate, or accuracy of a PCR … View Doc

Risk Of Missed PRRS PCR Detection – Wikipedia, The Free …
Real-time PCR using Taq-man chemistry is prone to false-negative results when the virus mutates. This specific source of the false-negative is not due to operator error on the part of the lab and is Given the rate of mutation for the PRRS virus, contingency plans should be developed for false … Read Article

Variants Of PCR – Wikipedia, The Free Encyclopedia
Faststart polymerase is a variant of Taq polymerase that requires strong heat activation, thereby avoiding non-specific amplification due Pfu DNA polymerase, isolated from the archean Pyrococcus furiosus, has proofreading activity, and a 5-fold decrease in the error rate of replication compared to Taq … Read Article

Manual: PfuUltra(tm) High-Fidelity DNA Polymerase
Owned by Hoffmann-La Roche Inc. and F. Hoffmann-La Roche Ltd. (“Roche”), has an up-front fee DNA polymerase exhibits an average error rate three-fold lower than PfuTurbo® DNA polymerase and 18-fold lower than Taq DNA polymerase, … Read Content

Easy-A High-Fidelity PCR Cloning Enzyme And Master Mix
3’-5’ proofreading activity and has been formulated to exhibit an error rate comparable to Pfu DNA polymerase* and 6-fold lower than Taq DNA polymerase1, 2 (see Table 1). Tgo DNA Polymerase (Roche)DNA Polymerase (Roche) 3.5 x 2.2 4.4 NR NR Vent® DNA Polymerase (New England BioLabs) 3.0 x 2.8 5 … Get Content Here

Photos of Roche Taq Polymerase Error Rate

Cancer Resequencing On Roche GS FLX
Cancer Resequencing on Roche GS FLX >> W orld' sH igh etF yDNA Pm a f CR The lower error rate and higher yields of K A PH iF ™ DN ® Taq High Fidelity. The lower error rate and higher yield of K AP H iF ™ l e a dstog nfc y p v Blend of wild-type Taq polymerase Single engineered polymerase … Retrieve Here

AmpliTaq Gold Polymerase, LD
Diagnostic uses require a separate license from Roche. Further information on purchasing licenses may be obtained by Polymerase, LD, carefully read “Preventing DNA Contamination” on page 2-2 before proceeding. … Return Document

In Diagnostic Procedures. FOR IN VITRO USE ONLY. Expand High …
Error rate1 3-fold more accurate compared to Taq DNA Polymerase Standard enzyme concentration 2.6 U (0.75 l) per 50 l reaction 2 Roche Applied Science 2. How To Use this Product 2.1 Before You Begin General considerations … Access Doc

Tgo DNA Polymerase
Www.roche-applied-science.com For life science research only. °C compared to Taq DNA polymerase with a half-life of † characterization of a population of cells in culture Enzyme Error Rate* … Access Full Source

Polymerase Chain Reaction – Wikipedia, The Free Encyclopedia
Can be utilized for PCR-mediated DNA mutagenesis, as higher Mn 2+ concentration increases the error rate A related patent battle over the Taq polymerase enzyme is still ongoing in several jurisdictions around the world between Roche and Promega. … Read Article

KAPA HiFi DNA Polymerase
Using a Roche 454 GS FLX next-generation DNA sequencer. 90 PCR amplicons, covering 5 candidate cancer genes from 24 error rate of KAPA HiFi is 100X lower than that of Taq Polymerase, 40X lower than that of polymerase blends such as Platinum® Taq High … Access Full Source

Tgo DNA Polymerase – New Thermostable Polymerase With …
Http://biochem.roche.com MOLECULAR BIOLOGY Background Information The major drawback of using Taq DNA Polymerase in an Table 1: Error rates of Tgo, Taq, and proofreading polymerases Enzyme Error Rate* Mutation Frequency** Taq DNA Polymerase 1.3 x 10-5 5.1% … Read Here

Taq DNA Polymerase
Error rate* approx. 1.3 ×10-5 Optimal enzyme concentration varies from 0.5-2.5 U per 50 µl reac- 7 Taq Polymerase: increased enzyme versatility in DNA Roche Applied Science offers a large selection of … Get Doc