Qiagen Taq Polymerase Error Rate

ProofStart PCR Handbook
DNA Polymerase or QIAGEN products in general, please do not hesitate to contact us. products with more than 10 times the accuracy of Taq DNA polymerase, providing the ideal tool for all PCR applications that require a low error rate, such as cloning and … Doc Viewer

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EN – Incubate 30 Min Add Aliquot To Competent Cells
ProofStart DNA Polymerase has an error rate more than 10 times lower than Taq DNA polymerase, making PCR products suitable ■ Issue No.2,2001 21 QIAGEN www.qiagen.com New ProofStart DNA Polymerase Figure 3 PCR was carried out using ProofStart … Retrieve Full Source

Qiagen Taq Polymerase Error Rate Photos

Journal Of General Virology (1998), 79, 2921–2928. Printed …
QIAgen microspin columns and sequenced by dye terminator cycle sequencing (Perkin-Elmer) on an Applied Biosystems 373 Published substitution error frequencies for Taq and Pfu expressed as errors per bp per cycle Polymerase error rate … Doc Retrieval

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Critical Evaluation Of Random Mutagenesis By error-prone …
The QIAquick MinElute Gel Extraction Kit (Qiagen). The gel-purified PCR products were cloned into pBADHisA cleaved with NcoI and XhoI. Error-prone PCR with Taq DNA polymerase and nucleotide analogues The error rate of Taq DNA polymerase was examined by increasing the proportion of … Retrieve Full Source

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Manual: Herculase Enhanced DNA Polymerase
As a result, the Herculase enhanced DNA polymerase can be used to successfully amplify small targets as well as genomic targets up to 37 kb and vector targets up to 48 kb, while maintaining a lower error rate than … Doc Retrieval

Product Insert SSP Page 1 Of 27 Instructions For Use SSP HLA …
However, this test has been replaced by polymerase chain reaction (PCR)-based DNA typing techniques due to its error rate and lack of allele hormone gene, PCR Master Mix including Taq polymerase (“Master Mix”), We recommend automated DNA extraction with the QIAGEN EZ1 DSP DNA … View Doc

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Optimized High-fidelity PCR With An Efficient 3' -> 5 …
QIAGEN www.qiagen.com rate (Figure 3). In contrast, Taq DNA poly-merase showed a relatively high error rate, and a Taq DNA polymerase/proofreading … Fetch This Document

Contribution Of Taq polymerase-induced errors To The …
Since Taq DNA polymerase substitution errors were surveyed by Saiki et al. (1988), new error-rate estimates have QIAgen microspin columns and sequenced by dye terminator cycle sequencing (Perkin-Elmer) on an Applied … Access Content

Qiagen Taq Polymerase Error Rate Images

Accuracy Of Replication In The polymerase Chain Reaction …
Columns (Qiagen, Chatsworth, CA). Se-quencing of 10 clones for each Taq or any significant difference in the error rate between Taq polymerase and ULTMA™ … Retrieve Content

Investigating The Effects Of DMSO On PCR Fidelity Using A …
Taq polymerase (Taq), which is commonly used in PCR, possesses no proof-reading ability and has an error rate of 10-4 base pairs, making PCR (Qiagen, CA), 0.25 mM dNTPs, 50 µM of each primer, 50 pg of pUC19 template DNA (Invitrogen, CA) and 1U of Taq polymerase … Return Doc

Taq polymerase – Wikipedia, The Free Encyclopedia
It lacks a 3' to 5' exonuclease proofreading activity, and has an error rate measured at about 1 in 9,000 nucleotides. The remaining two domains however In December 1999, U.S. District Judge Vaughn Walker ruled that the 1990 patent involving Taq polymerase was issued, in part, on misleading information … Read Article