Qiagen Hotstart Taq Polymerase

Influence Of Disk Separation Distance On Accuracy Of The Disk
2 (final concentration, 4.5 mM), 0.5 U QIAGEN HotStart Taq polymerase, and water to make a final volume of 25 l. Cycling conditions were 95°C for 15 min and then 35 cycles of 94°C for 30 s, 60°C for 30 s, … Read Document

Increased Specific Amplification And Erick B. Hu, Flexibility …
Platinum® Taq DNA Polymerase High Fidelity is a hot-start enzyme mixture composed of recombinant Taq DNA poly- Qiagen Proofstart™ Sigma JumpStart AccuTaq™ Taka ra Ex TaqHotStart Novagen … View Document

HotStart-IT : A Novel Hot Start PCR Method Results In …
HotStart-IT® has been added to USB FideliTaq™, which is a mixture of Taq DNA polymerase and a minor amount of a proofread- HotStarTaq is a trademark of Qiagen. ROX is a trademark of Applera Corporation or its subsidiaries in the U … Access Doc

Functional Genomics Of PycR, A LysR Family Transcriptional …
Containing PCR buffer (Qiagen), 200 mM dNTPs and 10 pmol of each primer with 2.5 U HotStart Taq DNA polymerase (Qiagen). TouchdownPCR(65–55 uC)wasperformed.ThePAO1DpycR::Gm … Get Doc

Isolation Of A Full-length CC NBS LRR Resistance Gene Analog
HotStart Taq polymerase (QIAGEN) in a 25-μlreaction. The cycling parameters consisted of a 15-min hot start at 94°C, followed by 12cycles of 94°C for 45s,62°C for 30s … Fetch Document

Materials And Methods
PCR amplification was performed in a 100-l volume using the HotStart Taq polymerase kit (Qiagen, Courtaboeuf, France), with 2 mM MgCl2, 100 µM of each of the four deoxyribonucleoside triphosphates (Roche Applied Science, Meylan, France), 0.25 M of the primers and 1.25 units of HotStartTaq DNA … Retrieve Content

Identification And Characterization Of A New Bocavirus …
With 5.2 ml106polymerase reaction buffer (Qiagen), 1.2 leach dNTP (10 mM), 20 pmol forward (GBoV-F1) and reverse primer (GBoV-R1), 1 ml HotStart Taq DNA polymerase … Get Document

Report On The Testing Of A PCR-based Detection Method For …
Detection method is sensitive to changes in Taq polymerase brand. The Limit of Detection (LOD) of the method has been estimated at 2500 copies – Qiagen HotStart Taq and Qiagen PCR buffer – 10mM dNTPs CRL-GMFF: Report on Florigene Moonlite 123.2.38 Carnation 5/15 … Read Full Source

BRCA1, BRCA2, TP53, And CDKN2A Germline Mutations In Patients …
CDKN2A and CDK4, the PCR mixture had 12 lM dNTP (Amersham Pharmacia Biotech), 1· PCR buffer (Qiagen), and 0.2 U Hotstart Taq polymerase (Qiagen). … Retrieve Doc

Four-Plex PCR amplification of amplicons containing variants of interest was performed using Qiagen HotStart Taq Polymerase on a Perkin Elmer GeneAmp 2400 thermal cycler with 5 ng genomic DNA in a 2.5 l reaction. … Document Retrieval