Promega Taq Polymerase Protocol

Polymerase Chain Reaction (PCR)
Note: different buffer receipes follow at the end of this protocol – MgCl 2 (25mM is convenient) – Taq polymerase – dNTPs (2mM stock) Note: a 2mM stock of dNTPs means Use of detergents is recommended only for Taq from Promega (up to 0.1% v/v, Triton X … Retrieve Full Source

Taq polymerase – Wikipedia, The Free Encyclopedia
Taq polymerase ( / ˌ t æ k ˈ p ɒ l ɨ m ər eɪ z /) is a thermostable DNA polymerase named after the thermophilic bacterium Thermus aquaticus from which it was The ruling supported a challenge by Promega Corporation against Hoffman-La Roche, which purchased the Taq patents in 1991. … Read Article

Polymerase Chain Reaction Optimization – Wikipedia, The Free …
Http://www.promega.com/pnotes/65/6921_27/6921_27_core.pdf. Polymerase errors. Taq polymerase lacks a 3' to 5' exonuclease activity. Thus, Taq has no error-proof-reading activity, which consists of excision of any newly misincorporated nucleotide base from the nascent (=extending) DNA strand that does not … Read Article

Rapid, High Throughput DNA Fragment Purification Using Wizard …
This simple modification of the protocol, coupled with the selectivity of silica Polymerase Storage Buffer A Polymerase Storage Buffer B Promega PCR Master Mix Perkin Elmer Ampli-Taq Gold … Retrieve Document

Version 5/4/99 California Institute Of Technology – Directed …
The protocol as described by Caldwell & Joyce uses pH 8.3. The Taq Promega buffer we 1 µl Taq polymerase (Promega) 2 µl primer 1(20 µM) 2 µl primer 2(20 µM) … Fetch Document

Efficiency Comparison Of Seven Different Taq polymerases Used …
(Sigma), Taq DNA Polymerase (New England BioLabs), and Taq DNA Polymerase (Promega). stepoftheinitialdenaturationaccordingtothemanufacturers’protocol. Primer sequences were marked with fluorescent dyes that enable detection … View Doc

Manual: PfuUltra(tm) High-Fidelity DNA Polymerase
PCR Protocol for PfuUltra™ High-Fidelity DNA Polymerase..10 in fewer mispaired primer-extension reactions than occur with Taq DNA polymerase during the lower temperatures encountered during PCR … View This Document

Polymerase Chain Reaction – Wikipedia, The Free Encyclopedia
A related patent battle over the Taq polymerase enzyme is still ongoing in several jurisdictions around the world between Roche and Promega. The legal arguments have extended beyond the lives of the original PCR and Taq polymerase patents, which expired on March 28, 2005. … Read Article

AFLP Protocol
This protocol is an amalgamation of protocols from Vos et al., Brent Barrett, Greg Penner, A.S. Reddy, Gibco Taq polymerase (5U/µl, Promega) 0.1 µl Total 20.2µl Run following PCR profile: … Doc Retrieval

PGem-T Clone Bank 1 …
By using Taq DNA polymerase to amplify the bacterial 16S rDNA. Mix: (amount for one PCR reaction) primer 8f (0.01mM stock from Promega, it is important that the appropriate transformation protocol be followed. … Access Full Source

| 1 Nucleic Acid Amplification – I. Introduction 1
As described in the Access RT-PCR Protocol using oligonucleotide primers specific to the mouse β-actin transcript. TaqDNA Polymerase FAQ (www.promega.com /faq/taq.html) GoTaq ® DNA Polymerase FAQ (www.promega.com … Return Doc

PCR Biotechnology
Set up reaction in accordance to manufacturer’s protocol for the taq (polymerase) being used. The taq used in this reaction was GoTaq green from Promega. … Access This Document

AmpliTaq 360 DNA Polymerase
Roche Taq DNA Polymerase New England Biolabs Taq DNA Polymerase Sigma REDTaq AmpliTaq® 360 DNA Polymerase Promega GoTaq® DNA Polymerase Stratagene Taq2000™ DNA Polymerase AmpliTaq® 360 DNA Polymerase Protocol 1 protocol 4398942 … Fetch Content