Polymerization Rate Of Taq Polymerase

From PCR To Electrophoresis
Is heat-degraded in a much lower rate as commonly used Taq DNA polymerase. Activity The enzyme has a 5’ g 3’ polymerization-dependent exonuclease replacement activity but lacks … Fetch This Document

Taq DNA Polymerase DNA-free
Order to initiate polymerization. The 5’ region of the primer is less sensitive to base mismatches. Error rate* approx. 1.3×10-5 Optimal enzyme concentration 0.5-2.5 U per 50 μl reaction Taq DNA Polymerase (5 U/μl) 0.4 μl 2 U/ reaction … Read Document

Manual: TaqPlus Precision PCR System
Also characterized by a relatively slow polymerization rate requiring the use of long extension times (i.e., ≥2 minutes/kb of DNA target) to generate high yields of PCR product.7 Taq DNA polymerase exhibits a fivefold higher … Access Document

PCR Guidelines And Optimization
At low Mg2+, Taq polymerase accuracy (correct complementary base pairing) is high, but polymerization rate is low. Conversely, at high Mg2+, the polymerization rate is high … Retrieve Full Source

Φ29 DNA polymerase – Wikipedia, The Free Encyclopedia
The polymerase catalyses the formation of the initiation complex between the terminal protein and two functional domains is essential, so as to ensure accurate elongation at an optimal rate. It is believed to be 1 or 2 orders of magnitude less error prone than Taq polymerase ; Generates large … Read Article

Thermophylic Polymerases A Users Guide Traditional Enzymes …
5’→■3’ polymerization Blends Wild Type Engineered Wild Type Wild Type Engineered Error rate [1], [2] Application Taq Polymerase ++ ++ 10-5 ■■ Standard PCR / optimized for minimal by-product formation … View Doc

Improvement Of 29 DNA polymerase Amplification Performance By …
And the 3′–5′ exonuclease domain of φ29 DNA polymerase, al-lowing the efficient coupling of processive DNA polymerization to strand displacement, neither impairing the polymerization catalytic rate nor the position of Taq DNA polymerase. Despite the fact that addition … Get Doc

KOD-plus Polymerase
Template-dependent polymerization of nucleotides into duplex DNA in the 5’=>3’ direction. The KOD DNA Polymerase also exhibits 3’=>5’ Eight times more accurate than Taq DNA polymerase. The error rate of KOD DNA Polymerase in PCR is 2.6×10-6 … Fetch Doc

Taq Full DNA Polymerase
Type Taq polymerase.) The Taq Full DNA polymerase has two catalytic activities: 1) It catalyzes the 5’ to 3’ polymerization of nucleotides into duplex DNA with a processivity of 50–60 nucleotides and an extension rate of about 75 nucleotides … Get Content Here

Archaeal DUTPase Enhances PCR Amplifications With Archaeal …
The enzyme’s relatively slow polymerization rate (550 vs. 2,800 nucleotides per min for Taq) and interference from its associated 3 -to5 -exonuclease activity (13). Improvements in the performance of PfuDNA polymerase have … View Doc

Polymerase Chain Reaction – Wikipedia, The Free Encyclopedia
Almost all PCR applications employ a heat-stable DNA polymerase, such as Taq polymerase, an enzyme originally isolated from the bacterium Thermus aquaticus. ions; generally Mg 2+ is used, but Mn 2+ can be utilized for PCR-mediated DNA mutagenesis, as higher Mn 2+ concentration increases the error rate … Read Article

Summer 2008, Vol. 3.2 Expressions NE
Increasing fidelity is for the polymerase to have 3´→5´ exonuclease activity, termed “proofreading”. Considering the complexity of DNA polymerization, Taq Taq. DNA Polymerase will misincorporate nucleotides at a particular rate leading to … Doc Viewer