Pmsf Taq Polymerase

Purification And Enzymic Properties Of Mot1 ATPase, A …
Was carried out under standard conditions using Taq polymerase, 10 fmol of NotI-linearized albumin; dsDNA, double-stranded DNA; PCR, polymerase chain reaction; PMSF, phenylmethylsulfonyl fluoride; Pol II, eukaryotic RNA polymerase II; SDS-PAGE, … Fetch Document

Alkaline Protease Production Of Bacillus Cohnii APT5
While 1 mM PMSF had no eff ect on the enzyme activity, a 10 mM concentration of PMSF slightly decreased the activity, producing a relative μM forward and 0.25 μM reverse primer, 1.25 U Taq polymerase (Fermentas), 200 ng genomic DNA, and … Fetch Full Source

In Vivo Regulation Of Hepatitis B Virus Replication By …
Mannheim) and contained 2.5 U of Taq DNA polymerase. HBV DNA and RNA analysis. 0.15 mM spermine–0.5 mM spermidine–0.85 mM DTT–0.125 mM PMSF con-taining leupeptin (0.5 mg/ml), aprotinin (1.0 g/ml), and pepstatin (1.0 … Fetch Full Source

In Vivo And In Vitro Ob Gene Expression And Leptin Secretion …
(1.5%), leupeptin (0.2 nm), PMSF (100 nm), vitamin E (4 mg/ml), pen icillin (100 U/ml), streptomycin (0.1 mg/ml) and, when indicated, the Taq polymerase. PCR conditions were a denaturation step at 95 C for 2 min followed … Doc Retrieval

Molecular And Biochemical Characterization Of A CNP-Sensitive …
, phenylmethylsulfonyl fluoride (PMSF), Trizma base, dithiothreitol (DTT), sucrose, 3- dCTP, and dGTP, 0.5 mg/ml BSA, 2.5 U of native Taq DNA polymerase (MBI Fermentas, Hanover, MD), and 10 pmol each … Access Full Source

69 DTT) containing protease inhibitors (2 mM Aprotinin, 0.2 mM PMSF) and round, each of PCR components including nucleotide (4 mM), primers (1 AM), Taq polymerase (1 U) or DNA template (100 ng) was added as indicated in each … Fetch This Document

Materials And Methods
PMSF Sigma, Deisenhofen, Germany Protease inhibitor coctail tablets, Complete Mini of Taq polymerase (InViTek), 0.2 mM dNTPs, 1.5 mM MgCl2, formamide (Table 2-2) … Retrieve Content

A Soluble 3-hydroxy-3-methylglutaryl-CoA Reductase In The …
Restriction enzymes, T4 DNA ligase, Taq polymerase and the Klenow fragment of DNA polymerase from Boehringer Mann- PMSF and leupeptin were from Boehringer Mannheim, and Aquasol-2 was from NEN–Dupont. … Return Doc

Human Aldolase A Natural Mutants: Relationship Between …
Taq polymerase, T4 DNA ligase, isopropyl b-D-thiogalactoside (IPTG), ampicillin, PMSF, and a-glycerol-phosphate dehydrogenase/triose phosphate isomerase (GDH/TIM) were from Roche Molecular Biochemicals. … Return Doc

(PMSF), Polyoxyethylenesorbitan Monolaurate (Tween 20), Bovine Serum Albumin Sucrose, Ponceau Red S Solution, Taq DNA polymerase, PCR primers, … Content Retrieval