Platinum Taq Polymerase Ta Cloning

Development Of A PCR-Based Assay For Detection …
Each primer, 0.5 U of Platinum Taq Polymerase (Invitro-gen), and 1–10 L of template DNA. use of the primers P-025/P-026 and cloned into the TA cloning vector. These primers are located upstream and … Read Content

Spectral Diversity Of Fluorescent Proteins
Consisted of 2 !l cDNA, 2 U Platinum Taq polymerase (Invitrogen), 1x Platinum Taq buffer, 0.2 !M of each Extraction Kit, Qiagen), cloned into a TOPO TA cloning vector (pCR2.1-TOPO, Invitrogen), and then sequenced. … Retrieve Full Source

Research Communication Identification, Classification, And …
DNA was cloned using the TOPO TA cloning system (Invitrogen), and the resulting bacterial colonies were GTCTGAGGCCAGAACAGTTC-3 ) with Platinum Taq High Fidelity DNA polymerase system (Invitrogen). … Return Doc

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Platinum Taq DNA Polymerase Platinum Taq DNA Polymerase High Fidelity Taq DNA Polymerase, Native Taq DNA Polymerase, Recombinant K450001SC TOPO TA Kit, TOP10 (Supply Center only) Zero Blunt TOPO PCR Cloning Kit with One Shot TOP10 Chemically … Get Doc

GeneBLAzer Kit を利用したフローサイトメーター …
Platinum® Taq DNA Polymerase High Fiderity 11304-011 100 U 12,500 pGeneBLAzer™ TOPO® TA Cloning Kit in vivo Detection 直送できます 12578-027 20 反応分 128,000 … Get Doc

Protocol Submitted With Peer-reviewed Article – SPLICE: A …
MM dNTP mix and 25 mM MgSO 4 (Cat# 71086-3; Novagen, Nottingham, 4 (Cat# 71086-3; Novagen, Nottingham, 4 UK) or Platinum Taq DNA Polymerase Kit, with Platinum Ta q DNA such as the inclusion of restriction enzyme recognition sites in the two outer (sense and antisense) primers for ease of cloning … Fetch This Document

DifferentialexpressionoftwocytochromeP450genesincompatible …
Included in a TOPO TA clonings for sequencing kit (Invitrogen, Carlsbad, CA, USA) rather than cloning Invitrogen Platinum Taq DNA Polymerase High Fidelity. In each reaction, the amount of DNA and … Read More

Cloning, Sequence Analysis And Heterologous Expression Of The …
PCR was performed with Platinum Taq Polymerase (Gibco, BRL, Inc.) at a ¢nal MgCl2 concentration of vectors and transformed into E. coli TOP10FP cells (TA Cloning Kit, Invitrogen, Carlsbad, USA). … Retrieve Content

HMP 3730 16S Protocol Version 1
Platinum Taq DNA Polymerase High Fidelity(5U/ul) 0.10ul (Invitrogen #11304) Typical TOPO TA cloning reaction: 16s PCR product (~75ng) X ul … View Document

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Taq DNA Polymerase, Recombinant K450001SC TOPO TA Kit, TOP10 (Supply Center only) Zero Blunt TOPO PCR Cloning Kit with One Shot TOP10 Chemically Competent Platinum Taq DNA Polymerase Platinum Taq DNA Polymerase High Fidelity … Retrieve Here

Pfu DNA polymerase – Wikipedia, The Free Encyclopedia
This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. As a result, Pfu is more commonly used for molecular cloning of PCR fragments than the historically Pfu DNA polymerase is hence superior to Taq DNA polymerase for techniques that … Read Article