Platinum Taq Polymerase Protocol

Eppendorf Mastercycler® Ep System* – Optimizing Fast PCR …
- was implemented as a 2-step protocol. The following configurations were tested: • Eppendorf HM: Mastercycler ep gradient S + HotMaster Taq Polymerase • Eppendorf IP: Mastercycler ep gradient S + Platinum … Get Content Here

First-Strand CDNA Synthesis
Platinum ® Taq DNA Polymerase with SYBR ® Green I fluorescent dye in a SuperMix formulation with uracil DNA glycosylase (UDG) and dUTP to Autoclaved distilled water to 18 µl 612 µl Protocol 1. Program the real-time instrument to perform a brief UDG … View This Document

Platinum® Quantitative PCR SuperMix-UDG
3In LightCyclerfi reaction, total units of PlatinumTaq DNA Polymerase are 1.2 (including 0.6 U in Platinum fi Quantitative PCR SuperMix-UDG) Protocol for Instruments Using PCR Tubes/Plates … Retrieve Doc

Product Information Last Updated: September 27, 2010 EvaGreen
DNA Polymerase . Manufacturer AmpliTaq Applied Biosystems Hot Start Taq . Fermentas Platinum Taq Invitrogen AccuPrime Taq with high fidelity Record fluorescence in FAM channel B. Fast Protocol . 2 . Set the cycling parameters as follows when Fermentas Hot Start Taq, Platinum Taq, AccuPrime Taq … Read Document

MEKK-1 PCR Protocol
MEKK-1 PCR Protocol ** 10X buffer, 50 mM MgCl2, and platinum taq from Invitogen Platinum Taq Polymerase Kit (cat # 10966-018) … Read Document

Reverse Transcriptase – Wikipedia, The Free Encyclopedia
In the fields of molecular biology and biochemistry, a reverse transcriptase, also known as RNA-dependent DNA polymerase, is a DNA polymerase enzyme that transcribes single-stranded RNA into single-stranded DNA. It also is a DNA-dependent DNA polymerase which synthesizes a second strand of DNA … Read Article

O P T I M A L I Z A T I O N O F P C R P R O T O C O L F O R T …
The PCR protocol optimised. Touch down PCR: Tm=63 °C in 5 cycles and 58 °C in 40 cycles Platinum. TM . Taq . DNA Polymerase. 1. DNA isolationDNA isolation … Read Content

PCR Tips And Tricks.ppt
Invitrogen Platinum® Taq DNA Polymerase Antibody Applied Biosystems AmpliTaq Gold® Chemical • No long initial denaturation step in cycling protocol • Can optimize magnesium – the buffers do not contain magnesium … Fetch Document

Reaction Conditions Used In The CBOL Plant Working Group …
RpoC1 (Protocol provided by Robyn Cowan,; Taq polymerase (Platinum Taq, Invitrogen) 2 units BSA 0.1mg/ml … Fetch Here

Platinum® SYBR® Green QPCR SuperMix UDG
SYBRfi Green I, 60 U/ml PlatinumTaq DNA polymerase, 40 mM Tris-HCl (pH 8.4), 100 mM KCl, 6 mM MgCl Protocol 1. Program the real-time instrument to perform a brief UDG incubation immediately followed by PCR amplification, as shown … Return Document

PCR 8/GW/TOPO TA Cloning Kit
Produce PCR product Produce PCR products using Taq polymerase and your own protocol. Note: For improved specificity and higher yields, we recommend using Platinum® Taq DNA Polymerase available from Invitrogen (see page x for ordering … Read Content