Platinum Taq Polymerase Proofreading

Pfu DNA polymerase – Wikipedia, The Free Encyclopedia
The main difference between Pfu and alternative enzymes is Pfu's superior thermostability and 'proofreading' properties compared to Taq DNA polymerase. … Read Article

ZeroBack Fast Ligation Kit
Generated using Taq DNA polymerase or other non-proofreading thermostable DNA polymerases are blunted in 5 2x Taq Platinum PCR MasterMix (with loading dye) 0.5 ml 5 x 1 ml KT204-01 … Retrieve Document

Consisted of 2 !l cDNA, 2 U Platinum Taq polymerase (Invitrogen), 1x Platinum Taq buffer, 0.2 !M of each PfuUltra Hotstart polymerase (Stratagene), a high-fidelity, proofreading polymerase. … Visit Document

Strategies 18 1 – Newsletter
The proofreading component found in competi-tors’ PCR systems and enzyme blends. PicoMaxx high fidelity PCR system, Platinum Taq DNA polymerase high fidelity, and Expand high fidelity PCR system. … Fetch Full Source

Reverse Transcriptase – Wikipedia, The Free Encyclopedia
Lastly, RNA polymerase II transcribes the proviral DNA into RNA which will be packed into virions. Reverse transcriptase has a high error rate when transcribing RNA into DNA since, unlike any other DNA polymerases, it has no proofreading ability. … Read Article

Any competing high fidelity polymerase, including Phusion in its GC Buffer, Invitrogen Platinum Taq High Fidelity proofreading polymerases is poor sensitivity due to damaged nucleotides and primer … Document Viewer

AcquiredResistancetoImatinibinGastrointestinalStromalTumor …
Insert was done separately with Platinum Taq (Invitrogen) and Pfu ultra high-fidelity DNA polymerase (Stratagene, La Jolla, CA) in order to exclude false-positive mutations due to errors in polymerase proofreading. … Read Document

Utilization Of Microsatellite Polymorphism For …
And 0.2 U of proofreading Taq polymerase (Platinum, Invitrogen) were per-formed in a Mastercycler ep thermal cycler (Eppendorf, France). The reaction … Access Doc

Analysis Of The Major Hexose Transporter Genes In Wine …
1 min, 55°C for 1 min, 68°C for 2 min, using Platinum Taq High Fidelity DNA Polymerase (Invitrogen, Carlsbad, CA). This polymerase has proofreading activity to reduce … Fetch Content

SuperScript™ III First-Strand Synthesis System For RT-PCR
Reaction, we recommend one of the following DNA polymerases: PlatinumTaq DNA Polymerase provides automatic hot-start conditions for increased fidelity and higher yields for targets up to 15 kb. • Platinumfi Pfx DNA Polymerase possesses a proofreading 3· to 5· … Retrieve Document

© Stratagene, An Agilent Technologies Company
The proofreading activity of this polymerase formulation is unique in that it does high-fidelity PCR cloning enzyme or Taq DNA polymerases from two commercially DNA Polymerase Platinum Toq 300 bp 96% 95% 96 96 … Document Retrieval

RESEARCH COMMUNICATION Distribution Of HPV-16 Intratypic …
The E6 gene was amplified using a proofreading High Fidelity Platinum Taq DNA polymerase (Invitrogen, Carlsbad, CA) and a pair of PCR primers that amplified a … Fetch Here