Platinum Taq Polymerase Error Rate

Pfu DNA polymerase – Wikipedia, The Free Encyclopedia
Commercially available Pfu typically results in an error rate of 1 in 1.3 million base pairs and can yield 2.6% mutated products when amplifying 1 Pfu DNA polymerase is hence superior to Taq DNA polymerase for techniques that require high-fidelity DNA synthesis, but can also be used in conjunction with … Read Article

Accuprime Technology For High-specificity Pcr Systems …
The most widely used DNA polymerase, Taq DNA polymerase, has been plagued with a high error rate of ness of Platinum® Taq DNA Polymerase High Fidelity. The easy-to-use AccuPrime™ Taq DNA Polymerase High Fidelity system … Fetch Here

Tgo DNA Polymerase – New Thermostable Polymerase With …
Table 1: Error rates of Tgo, Taq, and proofreading polymerases Enzyme Error Rate* Mutation Frequency** Taq DNA Polymerase 1.3 x 10-5 5.1% Other proofreading 1.8 x 10-5 – 5.1–0.3% … Document Retrieval

Platinum PfxDNA Polymerase
How does Platinum® Pfx DNA Polymerase fidelity compare to other DNA polymerases? We determined the fidelity of leading DNA poly-merases using the rpsL Fidelity Assay described below (1). Table 1 shows the average mutation frequency and average error rate for Taq DNA … Return Document

AccuPrime Pfx : High-fidelity DNA polymerase With A Zing
Widely used DNA polymerase, Taq DNA polymerase, has been plagued with a high error rate at about 2 Platinum ® Pfx DNA polymerase and com plements AccuPrime ™ Taq DNA polymerase in generating a premier, next … Access Doc

PCR Core Systems Taq DNA Polymerase Pfu
Platinum ® Taq Tfl Tth (Tli) Vent ® Pfu >95% >95% Resulting DNA ends 3′ A3′ A3′ A3′ A Blunt Blunt Blunt fidelity of the DNA polymerase and lead to a higher error rate. Taq DNA polymerase is commonly supplied with buffers … Retrieve Full Source

Post-mortem DNA Damage Hotspots In Bison Bison Bison …
polymerase (Platinum Taq Hi-Fidelity, Invitrogen, UK) was used to reduce the polymerase error rate, and increase amplification efficiency [10,14,39]. … Fetch This Document

Pictures of Platinum Taq Polymerase Error Rate

KAPA HiFi DNA Polymerase
Calculated at 1 error in 8.9 x 104 bases covered (1.11 x 10-5). The error rate of KAPA HiFi is 100X lower than that of Taq Polymerase, 40X lower than that of polymerase blends such as Platinum® Taq High … Access Full Source

High Fidelity And High Specificity–
AccuPrime™ Pfx DNA Polymerase incorporates: • Pfx DNA Polymerase1,2,14,136—a thermostable proofreading enzyme with extremely low error ratePlatinum® “hot start” anti-Pfx Enzyme Taq Platinum® Taq Platinum® Pfx AccuPrime™ Pfx Buffer Strength 1x 1x 1x 2x 1x 2x Error Rate* 4.4 x 10-5 4.4 x 10-5 3.2 x … Read More

Easy-A High-Fidelity PCR Cloning Enzyme And Master Mix
Platinum®Pfx DNA Polymerase (Invitrogen)DNA Polymerase (Invitrogen) 2.3 x 3.5 7 35 70 Platinum®Taq DNA Polymerase High-Fidelity (Invitrogen)DNA Polymerase High-Fidelity (Invitrogen) 1.4 x 5.8 11 57 100 Taq DNA PolymeraseDNA Polymerase 1.0 x 8.0 16 80 NR Table 1: PCR Error Rate of Easy-A™ PCR Cloning … Get Document

Cancer Resequencing On Roche GS FLX
® Taq High Fidelity. The lower error rate polymerase Total Bases Sequenced 17,724,794 7,273,424 Bases Amplified/PCR Error 3,544,959 89,795 Error Rate 2.82 E-07 1.11 E-05 2.50 E-5 2.4 E-5 2.00 E-5 1.50 E-5 1.1 E-5 1.00 E-5 0.50 E-5 7.6 E-7 4.4 E-7 2.8 E-7 0.00 E-5 Taq Platinum HiFi PfuUltra Phusion … Visit Document

Reverse Transcriptase – Wikipedia, The Free Encyclopedia
It also is a DNA-dependent DNA polymerase which synthesizes a second strand of DNA complementary to the reverse-transcribed single-stranded cDNA after This high error rate allows mutations to accumulate at an accelerated rate relative to proofread forms of replication. … Read Article