Pfuultra Taq Polymerase

Gene Synthesis Using DNAWorks
10 μM 4 μl 3' primer 0.2 mM each 2.5 mM each 8 μl dNTPs ? ? 2 μl PCR mix hold 4°C 1X 5 ' 72°C 0.5 ' 72°C 20X 0.5 ' 62°C 0.5 ' 95°C 1X 2.0 ' 95°C p = mutation rate / 1000 nt / duplication (Cline et al., Nucleic Acids Res 24 (1996)) Taq polymerase = 0.008 KOD (Novagen) = 0.0027 PfuUltra … Read Here

Maximum Speed, Maximum Fidelity
PrimeSTAR® Max DNA Polymerase is a unique DNA polymerase based on the original PrimeSTAR HS, a non-Taq polymerase with high proofreading activity. Enzyme PrimeSTAR GXL PfuUltra™ II Fusion HS DNA Polymerase Pfx50™ DNA … Retrieve Full Source

Universal Cloning Kit 14-10-11
PCR System, Platinum® Taq High Fidelity and AccuTaq® DNA polymerase and second generation thermostable high fidelity DNA polymerases like Phusion®, iProof™, KAPAHiFi™ and PfuUltra™ DNA polymerases. … Read Document

Engineering Of Glycerol Dehydrogenase For Improved Activity …
Markers, and Taq DNA polymerase were from New England Biolabs (Ipswich, MA, USA). PfuUltra High Fidelity DNA polymerase was purchased from Stratagene … Retrieve Full Source

Expression,Processing,andGlycosaminoglycanBinding …
InusingpfuUltra.Usingtheforwardprimerforthe5 -halfand the reverse primer for the 3 -half, we then amplified the entire 315-HARE encoding cDNA (35 cycles, T m 55 °C, extension time 8 min). Immediately following the amplification, 2.5 units of Taq polymerase and 2 mM MgCl … View Doc

Engineering Platforms For Directed Evolution Of Laccase From …
,5 l Taq polymerase buffer, and 2.5 units of Taq polymerase.ThePCRcyclesfollowedwere94°Cfor5min,55°Cfor5min, (0.25mMeach),3%DMSO,and2.5unitsofPfuUltraDNApolymerase. The PCRs were performed as follows: 95°C for 2 min (1 cycle); 94°C for … View This Document

PCR Tools Novagen ®
Summary We have demonstrated that KOD Hot Start DNA Polymerase is a superior proofreading DNA polymerase capable of generating high yields of PCR amplicons and exhibiting very high fidelity, comparable to that of PfuUltra DNA Polymerase. By using KOD Hot Start DNA Polymerase in place of Pfu or Taq … Fetch Content

Yeast Orthologues Associated With Glycerol Transport And …
These were performed using 100-200 ng of genomic DNA as template, with 1,5 to 3 mM magnesium chloride, 0,2 mM dNTP mix, 0,5 µM of each primer, 1 U Taq DNA polymerase (Fermentas) or 2U of PfuUltra TM High-Fidelity DNA Polymerase (Stratagene). … Return Document

Templates with overlap primers (designed as described in the section entitled Design of overlap primers) and PfuUltra ® II Fusion Hot Start polymerase (Stratagene, La Jolla, CA, USA), gel-purified, and quantitated. The use of a high-fidelity polymerase reduces errors, but Taq polymerase PCR … Read Content

Consisted of 2 !l cDNA, 2 U Platinum Taq polymerase (Invitrogen), 1x Platinum Taq buffer, 0.2 !M of each PfuUltra Hotstart polymerase (Stratagene), a high-fidelity, proofreading polymerase. … Document Viewer