Pfu Turbo Taq Polymerase

The Yersinia Pestis Autotransporter YapC Mediates Host Cell …
System (Roche) or Pfu Turbo Taq Polymerase (Stratagene), respectively. PCR products and plasmid pMMB207 (Morales et al., 1991) were cut by appropriate enzymes, gel purified, and the PCR … Doc Viewer

PfuTurbo DNA Polymerase
Pfu DNA polymerase 1.3 × 10–6 2.6 Taq DNA polymerase 8.0 × 10–6 16.0 PLATINUM® Pfx 3.5 × 10–6 5.6 VentR ® DNA polymerase 2.8 × 10–6 5.6 … Access Full Source

A Mutant Pfu DNA polymerase Designed For Advanced Uracil …
Proof-reading Taq polymerase (Taq) and the proof-read-ing polymerase PfuCX (Stratagene), both which suffer 13-cycle protocol with Pfu-Turbo or PfuX7. As expected, only PfuX7 was able to amplify DNA isolated … Get Content Here

Pfu DNA polymerase – Wikipedia, The Free Encyclopedia
Pfu DNA polymerase is hence superior to Taq DNA polymerase for techniques that require high-fidelity DNA synthesis, but can also be used in conjunction with Taq polymerase to obtain the fidelity of Pfu with the speed of Taq polymerase activity. … Read Article

Images of Pfu Turbo Taq Polymerase

Manual: PfuTurbo DNA Polymerase
#600256 PfuTurbo DNA polymerase (2.5 U/ μl) 100 U 500 U 1000 U 5000 U 10× Cloned Pfu DNA polymerase reaction buffer reactions than occur with Taq DNA polymerase during the lower temperatures encountered during PCR cycling (e.g., primer annealing). … Get Document

Platinum DNA Polymerase High Fidelity Cat. Nos. Size Conc. 5 …
Platinum ® Taq DNA Polymerase High Fidelity Cat. nos. Size Conc. 5 U/μL 11304-011 100 reactions Store at −30°C to −10°C 11304-029 500 reactions … Doc Viewer

Manual: PfuTurbo Hotstart DNA Polymerase
Life of Pfu DNA polymerase is approximately 19 hours at 95°C. Unlike Taq DNA polymerase, denaturing temperatures up to 98°C can be used successfully with PfuTurbo hotstart DNA polymerase to amplify GC-rich … Fetch Doc

Polymerase Chain Reaction
You need for PCR Taq: old faithful, the original PCR polymerase now comes in every possible flavor High-fidelity: stays on the DNA better and has proofreading activity, so it amplifies longer templates with less errors (up to 12 kb) [examples: Taq HiFi, Pfu Turbo] “Hotstart”: polymerase is … View Doc

PowerPoint Presentation
Extension 68˚C or 72˚C: The polymerase copies the template adding the dNTPs from 5’ to 3’. PCR 0.1-5 ng plasmid DNA and 0.1-1 ug for genomic DNA 200 µM dNTPs (final concentration) 1-1.5 U Taq polymerase and Pfu Turbo polymerase 1.25-2.5U 0.1-1 µM of each primer fwd/rev 1-4 mM MgCl2 … Access Doc

Pfu Turbo Taq Polymerase Images

PfuTurbo DNA Polymerase
Pfu DNA polymerase is a highly thermostable enzyme, retaining 94–99% of Consequently, hot start techniques, which are commonly used with Taq DNA polymerase to improve product yield and specificity, generally are not … Retrieve Document

T O POcloning PC Rcloning Fora 50m Lreactionusing Stratagene …
TOPO cloning PCR cloning For a 50 mL reaction using Stratagene Pfu Turbo DNA Polymerase (cat# 600250, blunt ends) : 1. 5 µ L 10x Reaction buffer 2 µ L dNTPs 5 mM each 1 µ L Taq DNA Polymerase 2. … Doc Viewer

Wikipedia:Reference Desk/Archives/Science/2008 May 9 …
1.10 CO2 of fans; 1.11 Sensitivity of Taq polymerase to heat Oh, and just a recommendation, regular Taq Pol is good, but I would suggest trying to use Pfu turbo or ultra. … Read Article