Pfu Taq Polymerase Promega

Polymerase Chain Reaction Optimization – Wikipedia, The Free …
Http://www.promega.com/pnotes/65/6921_27/6921_27_core.pdf. Polymerase errors. Taq polymerase lacks a 3' to 5' exonuclease activity. form of Thermococcus kodakaraensis KOD1; Vent, which is extracted from Thermococcus litoralis; Pfu DNA polymerase, which … Read Article

Comparison Data For Taq Master Mix (1)
Taq DNA Polymerase. α-Taq DNA Polymerase Pfu DNA Polymerase Hotstart Taq DNA Polymerase maker GeneAll Promega TaKaRa Stratagene product name. Pfu. Pfu Pyrobest Pfu … Retrieve Doc

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PCR Core Systems Taq DNA Polymerase Pfu
Www.promega.com • techserv@promega.com 13 Amplifying DNA 4375MA11_3A PCR Core Systems Taq DNA Polymerase Pfu DNA Polymerase Tli DNA Polymerase GoTaq … View Document

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TA Cloning Using Pfu, Taq, And TOPO Cloning Kit
Perform PCR reaction as normal using Pfu polymerase—if did PCR using Taq skip to step 4. Gel purify PCR fragment (used Montage kit, but others probably will work fine) “Tail” the purified PCR fragment: (adapted Promega pGEM cloning kit) … Read Content

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Platinum DNA Polymerase High Fidelity Cat. Nos. Size Conc. 5 …
Platinum ® Taq DNA Polymerase High Fidelity Cat. nos. Size Conc. 5 U/μL 11304-011 100 reactions Store at −30°C to −10°C 11304-029 500 reactions … View Document

Pfu Taq Polymerase Promega Pictures

Manual: Pfu DNA Polymerase
Product obtained using Pfu DNA polymerase. For Taq DNA polymerase-based PCR amplifications, an extension time of 0.5-1.0 minute/kb of template amplified is usually sufficient for maximum synthesis of a PCR … Fetch Doc

Pfu Taq Polymerase Promega Photos

Basic PCR Protocol (adapted From Promega Corp
Promega's Taq DNA Polymerase in Storage Buffer A requires. the use of Promega's 10X Reaction Buffer, or a very similar buffer Taq/ AmpliTaq Tfl Stoffel Frag. Tth VentR (exo-) VentR/ Tli Deep VentR Pfu Pwo UITmaT 5´-3´ Exonucl. … Fetch Content

Pfu Taq Polymerase Promega Photos

Manual: PfuUltra(tm) High-Fidelity DNA Polymerase
Pfu DNA polymerase is a highly thermostable enzyme, retaining 94–99% of its polymerase activity after 1 hour at 95°C. Unlike Taq DNA polymerase, … Get Content Here

Pfu Taq Polymerase Promega Photos

Promega Notes: Pfu DNA Polymerase: A High Fidelity Enzyme For …
Corresponding author: e-mail to mslater@promega.com Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied (1-3). For routine PCR, where simple detection of an amplification product or estimation of the product's size is important, Taq DNA polymerase is the … Retrieve Doc

PCR Tips And Tricks.ppt
TM* Pfu* Pwo*UITmaTM* 5'-3' Exonuclease Activity Yes No No No No No No 3'-5' Exonuclease • Embed Taq DNA polymerase or Mg in Wax Promega’s TaqBead™ Hot Start Polymerase (M5661) • Antibody inhibition of Taq DNA polymerase … Return Document

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Week 5 PCR Hotstart Pfu
The DNA polymerase isolated from Pyrococcus furiosis possesses what nuclease activity that is missing from Taq DNA polymerase? 8. What is the main advantage of Pfu over Taq in PCR? 9. Does Pfu have terminal transferase-like activity as Taq does? 10. Why does Promega recommend using hot start PCR … Fetch Full Source