Pfu Taq Polymerase Invitrogen

TOPO Cloning – Wikipedia, The Free Encyclopedia
Different types of vectors are used for cloning fragments amplified by either Taq or Pfu polymerase as Taq polymerase (unlike Pfu) leaves an extra "A" nucleotide at the 3'end during amplification. … Read Article

Amplification Efficiency Of Thermostable DNA polymerases
Cloned Pfu, PfuTurbo, PfuTurbo Hotstart, PfuUltra, Herculase, Herculase Hotstart, and SureStart Taq DNA polymerases were from Stratagene. Platinum Taq and Platinum Pfx were purchased from Invitrogen. Tgo DNA polymerase was purchased from Roche Molecular … Access Document

DNA Polymerase
1/2 DNA Polymerase The Secret of Life DNA polymerase plays the central role in the processes of life. It carries the weighty responsibility of duplicating our genetic … View Full Source

BioMed BMC Biotechnology Open Access Methodology Article
Vector NTI (Invitrogen) choosing Tm values around 55°C (probe 250 pM, salt 50 mM) or with the primer selection Biolabs) or a mixture of Pfu polymerase and Taq polymer ase (2.5 U Pfu + 1 U Taq per 50 µl PCR reaction) in Pfu … Read More

PowerPoint Presentation
PCR 0.1-5 ng plasmid DNA and 0.1-1 ug for genomic DNA 200 µM dNTPs (final concentration) 1-1.5 U Taq polymerase and Pfu Turbo polymerase 1.25-2.5U 0.1 The 3’-end of the primer has to end on an C or a G. 5’ 5’ 3’ 3’ * * Primer design for site-directed mutagenesis Invitrogen: Only … Content Retrieval

TA Cloning Kit
And Pfu, do not leave 3· A-overhangs. PCR products generated with Taq polymerase have a • Using Platinum fi Taq DNA Polymerase High Fidelity (Invitrogen, Catalog no. 11304- … Visit Document

Semiquantitative RT-PCR Analysis
The PCRs were performed using Pfu turbo DNA polymerase (STRATAGENE) for p15INK4b and platinum Pfx DNA polymerase (Invitrogen) for p19INK4d with the KpnI site-conjugated primers. The isolated DNA was amplified by PCR using an AmpliTaq Gold DNA polymerase (Applied Biosystems) or an LA Taq DNA polymerase … View Doc

MIH Quotation From BIOLINE Quote Number Competent Cells Are …
BIOTAQ DNA Polymerase BIO-21040 500 Units $110 Standard Taq polymerase 22c/unit (less if bought in bulk Pfu DNA Polymerase BIO-21101 250 units $170 Pfu, Phusion, Vent SensiMix SYBR One-Step QT245-02 250 reactions $1,075 Invitrogen One StepqRT-PCR kit One Step qRT … Retrieve Doc

Invitrogen Platinum® Taq 30 sec–2 min, 94°C Ab OneTaq™ DNA Polymerase M0480S/L/X 200/1,000/5,000 units £35/£ Phusion High-Fidelity DNA Polymerase from NEB – the enzyme that puts fidelity first from the company that puts science first. Phusion Pfu Taq … Document Retrieval

Development Of A TaqMan –Minor Groove Binding Protein Assay …
The LOD of PFU of virus was established using IbAr 10200 RNA extracted from cell culture amplified from total viral RNA using the Superscript™ One-Step RT-PCR with Platinum Taq kit (Invitrogen). by 1 cycle at 95°C for 2 minutes to inactivate the SSII RT and activate the Platinum Taq polymerase … Fetch Content

The Next Generation In Hot Start PCR Product Manual
DNA Polymerase Vendor pH/Reaction Units/µL Taq Invitrogen 8.4 5.0 Taq New England Biolabs 8.3 5.0 Taq USB 8.6 5.0 Taq Enzymatics 8.3 5.0 Pfu Stratagene 8.8 2.5 … Retrieve Content