Optimal Ph Taq Polymerase

COA: Taq DNA Polymerase, #EP0072
67 mM Tris-HCl (pH 8.8 at 25°C), 6.7 mM MgCl2, 1 mM 2-mercaptoethanol, 50 mM NaCl, 0.1 mg/ml BSA, The optimal extension temperature for Taq DNA Polymerase is 70-75°C. The recommended extension step is 1 min at … Access Doc

FS Taq DNA Polymerase TM FS
20 mM Tris-HCl(PH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5 %TW 20, 0.5 % NP 40, 50 % Glycerol Optimal reaction conditions (incubation time and temperature, concentration of FSTM Taq DNA Polymerase, primers, Mg2+, and template DNA) … Retrieve Full Source

Precision™ Taq DNA Polymerase
50mM Tris-HCl (pH 8.2 at 25°C), 0.1mM EDTA, 1mM DTT, 0.05% CHAPS and 50% glycerol. The following basic protocol serves as a general guideline and a starting point for any PCR amplification. Optimal reaction conditions (incubation times and temperatures, concentration of Taq Polymerase, primers … Fetch This Document

Taq DNA Polymerase
Enzyme is supplied in 20 mM Tris-HCl pH 8.5, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20 , 0.5% NP40, 50% glycerol. Quality Control Each lot of Taq DNA Polymerase is Suggested Protocol using Taq DNA Polymerase This protocol serves as a guideline for primer exten-sions. Optimal reaction … Doc Viewer

Polymerase Resistance To Polymerase Chain Reaction Inhibitors …
Kenneth D. Eilert,1, M.S. and David R. Foran,2 Ph.D. Polymerase Resistance to be optimal when working with DNA from skeletal remains. KEYWORDS: forensic science, polymerase, polymerase chain reaction, inhibition, inhibitors Taq polymerase reverses inhibition of quantitative real time polymerase … Retrieve Full Source

Optimization Of The
Of dNTPs, pH, and magnesium were tested iteratively, and the optimal con- ditions with regard to these variables have been defined. We have confirmed the finding of Eckert and Kunkel(7, 9) and defined conditions in which the Taq polymerase operates with a three- … View This Document

PCR Optimization: Reaction Conditions And Components
Optimal annealing temperatures and primer concentrations must be determined empirically. Taq DNA Polymerase has activity in the 25 °C-72 °C range. Gold® DNA Polymerase is 0.15% NP-40, 0.15% Tween 20, 0.1 mM EDTA and 25 mM Tris-HCl (pH 8.3 at room temperature). … Fetch Full Source

Prime Taq Premix (2X)
Prime TaqTM DNA Polymerase 1 unit/10 μl, 20 mM Tris-HCl, 80 mM KCl, 4 mM MgCl2, enzyme stabilizer, sediment, loading dye, pH 9.0, 0.5 mM of each dATP, dCTP, dGTP, dTTP Cat. No. G-2000 1.0 mL X 1 G-2001 1.0 mL X 3 G-2002 1.0 mL X 5 USAGE INFORMATION Optimal reaction … Visit Document

EconoTaq® DNA PolymeraseTaq DNA Polymerase
˚ Performance, Value and Convenience Master Mix Components. 0.1 unit/μl of Taq DNA polymerase, Reaction Buffer (pH 9.0), 400 μM dATP, 400 μM dGTP, 400 μM dCTP, 400 challenging GC-rich templates others cannot (Figure 1). •• 2X Master Mix contains a proprietary enhancer, dNTPs, and optimal … Get Document

Taq-red™ DNA Polymerase
Optimal reaction conditions (incubation times and temperatures, concentration of Taq Polymerase, primers, MgCl2 and template 500mM KCl, 100mM Tris-HCl (pH 8.0), 25mM MgCl2 and enzyme stabilizers. Unit Definition … Get Document

Psychrophile – Wikipedia, The Free Encyclopedia
Halobacterium; Helaeomyia petrolei; Hydrothermal vent; Methanopyrus; Movile Cave; Radiotrophic fungus; Rio Tinto; Taq polymerase; Thermostability; Thermotogae … Read Article