Metabion Taq Polymerase

High-Fidelity In Vitro Recombination Using A Proofreading …
Metabion GmbH (Martinsried, Ge- r many). Plasmid preparations were pe-r 2.5 U Vent DNA polymerase were used. The PCR protocol consisted of an initial denaturation step for 5 min at … Retrieve Content

Simple Technique For Internal Control Of Real-Time …
ICOs were synthesized by Metabion. (Note: To avoid contamination it is important not to order ICOs at initial denaturation and activation of Taq polymerase, followed by 50 thermal cycles of 95 °C for 10 s, 55 °C for … Retrieve Full Source

Rapid Second-Tier Molecular Genetic Analysis For Congenital …
All oligonucleotides were synthesized by Metabion. dna extraction DNA was extracted from three Guthrie card punches tion buffer, nucleotides, and Taq polymerase; Roche Mo lecularBiochemicals),and2.5 LofDNA.Thethermocy … Fetch Document

Characterization Of The 3-HKT Gene In Important Malaria …
(Metabion, Germany), 10X buffer, 3 units Taq DNA polymerase (Finnzymes, Finland) and 10 ng template DNA made up with de-ionized water. The reaction was … Fetch Document

Assessment Of Genetic Variations In Some Vigna Species By …
Primers (Metabion International AG. ) in a 25µL vol ume, containing (5 µL of 5x buffer, 3.0 µL of dNTPs (2.5mM ) 3 µL of Mgcl2 (25 mM), 3.0 µL primer (2.5 µ L ), 0.3 µ L of Taq polymerase ( 5U/ µL) and 2.0 … Read Here

Nature And Science, 2009;7(5), ISSN 1545-0740, Http://www …
The primers used (Metabion international AG, Deutschland) to detect the Ki-ras gene, were as follows: containing 2.5 units of Taq polymerase, 100 pmol of each primer, deoxyribonucleoside triphosphates ( … Fetch Full Source

Genetic Variability Of Czech And German RHD Virus Strains
Centration each (Metabion GmbH, Germany), 1.0 µl of dNTPs blend at 10 mM concentration (Promega, USA), 5.0 µl of 10-fold concentrated PCR buffer, 1.0 µl of Taq DNA polymerase, 1.0 µl of 10-fold … View Full Source

Materials And Methods
Fragments for pyrosequencing were generated by PCR using the FastStart Taq™ DNA Polymerase Kit (Roche, Basel, Switzerland) with the following protocol: 400 nmol/l of forward and reverse PCR primers (Metabion, Martinsried, Germany), 200 or 400 nmol/l of each dNTP, 2 mmol/l MgCl2, and 1.5 units … Read More

Development Of A Single Tube 640-plex Genotyping Method For …
Taq DNA polymerase (Solis Biodyne), 40mM universal primer (5 0-GATCAGGCGTCTGTCGT GCTC-3 0) (Metabion Int. AG). PCR ampliļ¬cation was conducted in a TProfessional Basic thermocycler under … Get Content Here

COMPARISON OF FOUR DNA EXTRACTION METHODS FROM INVASIVE …
1. mi-Tissue Genomic DNA Isolation Kit (Metabion GmbH, Martinsried, Germany ) dNTPs, 0.2µM of each primer and 1 unit of Taq polymerase (Fermentas UAB, … Fetch Content