Mbi Fermentas Taq Polymerase Buffer

Evaluation Of Three Methods For DNA Fingerprinting Of …
2.5 µl 10×PCR buffer, 3.5 mM MgCl 2, 0.2 mM of each dNTP (MBI Fermentas), 20 pmol of primer OPA03, 30 ng DNA template, 1 µl BSA (20 mg/ml), 1 U of Taq polymerase (MBI Fermentas) and water to 25 µl. … Get Document

EST-based Identification Of Genes Expressed In The …
0.5 U Taq polymerase (MBI Fermentas), 5mM of each dNTP, 1× buffer for Taq polymerase (MBI Fermentas). Each PCR cycle included denaturation at 95°C for 40s, … View Full Source

Full Length Article PCR Studies On Genetic Diversity Of …
Unit) Taq polymerase and 8.3 µL dH2O. Taq polymerase together with buffer, MgCl2 and dNTPs were purchased from MBI, Fermentas. Amplification was performed in … Document Retrieval

Molecular Cloning And MRNA Expression Pattern Of Sox9 During …
Template, 1 μl each primer (100 mM), 1 U Taq polymerase (MBI, Fermentas), 1μldNTP(10mMeach),5μl 10× buffer for Taq polymerase (MBI, Fermentas). … Fetch This Document

Visualization Of Symbiotic Tissue In Intact Root Nodules Of …
Reaction contained 1 × Taq polymerase buffer (MBI Fermentas) resulting in 10 mmol/L Tris-HCl (pH 8.8), 50 mmol/L KCl and 0.08 % Nonidet (nonionic detergent) with 1.25 mmol/L MgCl2, 0.15 mmol/L dNTP, … Get Content Here

Disease Of Aquatic Organisms 83:37
25 µl reaction mixture comprisi ng 1 µl of extracted genomic DNA, 5 µl of 1 mM deoxyribonucleotide triphosphates (dNTPs, MBI Fer-mentas), 0.25 µl of each primer (50 pmol µl-1), 2.5 µl of 10× Taq buffer (MBI Fermentas), 2 µl of 25 mM MgCl 2, 1 µl of Taq polymerase (2 U) (MBI Fermentas) and 13 … Document Viewer

The Relationship Between The Response To Risperidone …
buffer with (NH4)2SO4 (MBI Fermentas, Vilnius, Lithuania) and 1.25 U Taq polymerase (MBI Fermentas). Amplification was performed on an … Fetch Full Source

Structure, Promoter, And Additional Polymorphisms Dieter …
Solution in a final volume of 50 mL with 2.5 U Taq polymerase and the standard PCR buffer (MBI Fermentas). Oligonucleotide primers were routinely used at 0.4 mmol/L each, and a 0.2 mmol/L concen … Document Viewer

Assessment Of Endophytic Bacterial Diversity Among Mangrove …
µmol, as well as 1 U of Taq polymerase and buffer (MBI Fermentas). After the initial denaturation for 3 min at 95°C, there were 40 cycles consisting of denaturation at 95°C for 1 min, … Document Retrieval

High Resolution Melting As An Alternative Method To Genotype …
Primer, 1× PCR buffer, 1IU Taq polymerase (MBI Fermentas, Germany) and 50 ng of genomic DNA template in a total volume of 25 µl. The HRM anal- … Read Full Source