Klen Taq Polymerase

A Quantitative Model Of Error Accumulation During PCR …
Structure/function relationships of T7 DNA polymerase, Klen-Taq DNA polymerase, and a Bacillus DNA polymerase which have been worked out in some detail (Johnson, 1993; Kiefer et … Doc Retrieval

Acetolactate Synthase Target-Site Mutations And Single …
Concentration of Klen Taq buffer, and 1.75 unit of Klen Taq LA polymerase.5 PCR was performed in a Mastercycler epGradient thermal cycler, under the following conditions: … Read Document

Rapid Species Identification Within The Mycobacterium …
Klen Taq polymerase (AB Peptides, St Louis, MO), 0.12 µg of TaqStart Antibody (ClonTech, Palo Alto, CA), and 1× LCGreen I (Idaho Technology, Salt Lake City, UT). … Return Document

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Klentaq278(M707) has approximately 88% of Klen taq278 thermostability. Tth polymerase is cha racterized with highest tolerance of Na, K, and Mg salts, followed by Taq and Tne. … Document Retrieval

Taq polymerase – Wikipedia, The Free Encyclopedia
Taq polymerase is a thermostable DNA polymerase named after the thermophilic bacterium Thermus aquaticus from which it was originally isolated by Thomas D. Brock in 1965. … Read Article

Two Novel Human X-linked Homeobox Genes,
IN) or Klen-Taq polymerase (AB Peptides, St. Louis, MO) in a GeneAmp PCR System 9600 thermacycler (Perkin Elmer, Foster City, CA). The primers used to amplify … Doc Retrieval

Two Functional Forms Of Vascular Endothelial Growth Factor …
Each pair of primers using Klen Taq DNA polymerase mix (CLON TECH). Following the digestion with XhoI and XbaI, the PCR products were cloned into the mammalian expression vector pcDNA3 (Invitro … Access Content

Maximizing The Efficacy Of Melt Profiling Through Stringent …
Real-time PCR is Extremely Useful to Assess Amplification Adapting a LightCycler Assay Reaction volume of 10μl Hot-start polymerase, Klen Taq in a complex with TaqStart antibody Add LCGreen to PCR mastermix (1μl/reaction) and omit an equal amount of water. … View Doc

Oligopeptide Transporter PepT1 In Atlantic Cod ( Gadus Morhua …
(Clontech, Mountain View, CA, USA) with Advantage Klen Taq polymerase (Clontech). PolyA(+) RNA was purified with the Oligotex mRNA Midi Kit (Qiagen GmbH, Hilden, … Access Content

Klenow Fragment – Wikipedia, The Free Encyclopedia
The Klenow fragment was also the original enzyme used for greatly amplifying segments of DNA in the polymerase chain reaction (PCR) process, before being replaced by thermostable enzymes such as Taq polymerase. … Read Article

-K -ATPase Gene Expression In The Avian Eggshell Gland …
Tage KlenTaq polymerase (PE Applied Biosystems, Foster City, CA). PCR primers were a pairwise combination of Taq DNA polymerase, and Taq buffer containing 1.5 mM MgCl 2. PCR was performed for 30 cycles (94°C for 20 s, 60°C … Access Full Source

Soldier Caste-specific Gene Expression In The Mandibular …
Klen Taq polymerase (CLONTECH). Primers used in the PCR wereadaptorprimer1includedintheCLONTECHkit(59-CCA TCC TAA TAC GAC TCA CTA TAG GGC-39) and specific … Read Content