Invitrogen Taq Polymerase Troubleshooting

PcDNA TA Cloning Kit Five-minute Cloning And Expression Of Taq
Taq DNA Polymerase High Fidelity available from Invitrogen (see page vi for ordering information). Troubleshooting Introduction The table below lists some potential problems solutions that may help you … Read Here

Taq DNA Polymerase, Recombinant
Taq DNA Polymerase, recombinant Cat. Nos. 10342-053 (100 units) 10342-020 (500 units) Critical parameters and troubleshooting information are documented in ©2003 Invitrogen Corporation. All rights reserved. … Retrieve Doc

Platinum Taq DNA Polymerase
Incorporation of modified Oligos into DNA by Taq Troubleshooting Nonspecific Bands After PCR (smearing) Platinum Taq DNA Polymerase Author: Invitrogen Technical Support Subject … Retrieve Content

Applications And troubleshooting
Troubleshooting is virtually impossible without any controls. To ensure the efficiency of the cloning reaction, each of Invitrogen’s kits includes controls. Compatibility of DNA Ends of Vector and Insert TA Cloning ® technology was designed to clone PCRproducts produced by Taq polymerase. … Content Retrieval

TA Cloning Kit
We recommend using Platinum® Taq DNA Polymerase High Fidelity available from Invitrogen (see page viii for ordering Refer to the Troubleshooting section, page 8 for additional tips. Long-Term … View Document

Taq PCR Handbook
TaqDNA Polymerase and HotStar HiFidelity DNA Polymerase for Amplification of Long PCR Products 24 Troubleshooting Guide 28 Taq DNA Polymerase, the Taq PCR Core Kit, and the Taq PCR Master Mix Kit are … Read Content

PBAD/TOPO Expression Kit – Catalog No. K370-01
For country-specific contact information visit our web site at www.invitrogen.com pBAD/TOPO ® ThioFusion ™ Expression Kit Five-minute cloning of Taq polymerase-amplified Troubleshooting … Access Full Source

Troubleshooting Guide For PCR
Taq DNA Polymerase (#EP0611), Maxima ™ Hot Start Taq DNA Polymerase (#EP0601) or PyroStart Fast PCR Please refer to the troubleshooting in chapter Molecu-lar Cloning (p.340). DNA polymerase present in digestion reaction mixture. … View Doc

TOPO Cloning – Wikipedia, The Free Encyclopedia
Different types of vectors are used for cloning fragments amplified by either Taq or Pfu polymerase as Taq polymerase (unlike Pfu) leaves an extra "A" nucleotide at the 3'end during amplification. … Read Article

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Www.invitrogen.com/cofa, and is searchable by product lot number, which is Taq DNA Polymerase Cat. Nos. 18038-018 (100 units) 18038-042 (500 units) Critical parameters and troubleshooting information are documented in … View Document

Primer (molecular Biology) – Wikipedia, The Free Encyclopedia
A primer is a strand of nucleic acid that serves as a starting point for DNA synthesis. They are required for DNA replication because the enzymes that catalyze this process, DNA polymerases, can only add new nucleotides to an existing strand of DNA. The polymerase starts replication at the 3 … Read Article

Real-time polymerase Chain Reaction – Wikipedia, The Free …
The close proximity of the reporter to the quencher prevents detection of its fluorescence; breakdown of the probe by the 5' to 3' exonuclease activity of the Taq polymerase breaks the reporter-quencher proximity and thus allows unquenched emission of fluorescence, which can be detected after … Read Article

PCR Protocol Using Taq DNA Polymerase
Taq PCR Handbook 10/99 11 Taq DNA Polymerase Protocol PCR Protocol Using Taq DNA Polymerase This protocol serves as a guideline for PCR amplification. … Return Document