Invitrogen Taq Polymerase Manual

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TA Cloning Kit
Type of Kits This manual is supplied with the following kits. Kit Quantity Catalog no. We recommend using Platinum® Taq DNA Polymerase High Fidelity available from Invitrogen (see page viii for ordering … Fetch Doc

Lot No.: Conc.: 5 U/ Non-frost-free Freezer. LICENSED FOR PCR
Associated with manual hot start procedures are addressed with the use of PLATINUM Taq DNA Polymerase. Licensed to Life Technologies, a Division of Invitrogen Corporation, under U.S. … View This Document

TA Cloning – Wikipedia, The Free Encyclopedia
It is best if the PCR primers have guanines at the 5' end as this maximizes probability of Taq DNA polymerase adding the terminal adenosine overhang. Thermostable polymerases containing extensive 3´ to 5´ exonuclease activity should not be used as they do not leave the 3´ adenine-overhangs. … Read Article

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Page 4 Of 4
Platinum® Taq DNA Polymerase 20 μL 50 μL 100 μL 1000 μL 10X PCR Buffer, Minus Mg 1.25 mL 1.25 mL 2.5 mL 50 mL Limited Use Label License No. 14: Direct Inhibition by Anti-Polymerase Antibodies Licensed to Invitrogen Corporation, under U.S. Patent Nos. 5,338,671; 5,587,287 … Fetch Content

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PcDNA 3.3-TOPO TA Cloning Kit
User Manual Corporate Headquarters Invitrogen Corporation 1600 Faraday Avenue Platinum® Taq DNA Polymerase High Fidelity available from Invitrogen (see … Read Full Source

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Taq PCR Handbook
Taq DNA Polymerase Kit 100 x 250 units Taq DNA 201209 (25000 U) Polymerase, 10x PCR Buffer,* respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user … Fetch Full Source

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TOPO TA Cloning®
For country-speciļ¬c contact information visit our web site at www.invitrogen.com TOPO TA Cloning® Five-minute cloning of Taq polymerase-amplified supplied with cat. nos. K4510-02 and K4500-02, refer to the manual supplied with … Retrieve Doc

TOPO TA Manual
polymerase, Taq must be used in excess of a 10:1 ratio to ensure the presence of TOPO TA Manual Author: Invitrogen Subject: TOPO Cloning with pCR2.1-TOPO and pCRII-TOPO … Fetch This Document

Primer (molecular Biology) – Wikipedia, The Free Encyclopedia
A primer is a strand of nucleic acid that serves as a starting point for DNA synthesis. They are required for DNA replication because the enzymes that catalyze this process, DNA polymerases, can only add new nucleotides to an existing strand of DNA. The polymerase starts replication at the 3 … Read Article

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PCR 8/GW/TOPO TA Cloning Kit
Recommend that you follow the detailed protocols provided in the manual. Step Action Produce PCR product Produce PCR products using Taq polymerase and your own using Platinum® Taq DNA Polymerase High Fidelity available from Invitrogen … View Document

Real-time polymerase Chain Reaction – Wikipedia, The Free …
The close proximity of the reporter to the quencher prevents detection of its fluorescence; breakdown of the probe by the 5' to 3' exonuclease activity of the Taq polymerase breaks the reporter-quencher proximity and thus allows unquenched emission of fluorescence, which can be detected after … Read Article

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PBAD/TOPO Expression Kit – Catalog No. K370-01
Taq DNA Polymerase available from Invitrogen (see page 32 for ordering information) to generate your PCR product. plasmid DNA for automated or manual sequencing, we recommend using Invitrogen’s PureLink … Retrieve Here

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250744C PENTR5'TOPO Man
Recommend that you follow the detailed protocols provided in the manual. Step Action Produce PCR product Amplify DNA encoding a eukaryotic promoter of choice using Taq polymerase and Taq DNA Polymerase available from Invitrogen (see page x for ordering information) … Access Full Source