Hotstar Taq Polymerase Qiagen

Eight New Dinucleotide Microsatellite Loci In Turkey …
2.5 pmol each primer, 100 mm dNTP, and 0.35 U Hotstar Taq polymerase (Qiagen, CA). Amplifications were performed in a MJ Research thermal cycler under the following reaction conditions: … Fetch Full Source

Macrophage-Derived Matrix Metalloproteinase-2 And -9 Promote …
Using the RNeasy Fibrous Tissue Mini Kit (QIAGEN). Extraction was performed according to the manufacture’s direction. By Sensis-cript reverse transcriptase (QIAGEN), total RNA was converted into cDNA, which was used in each PCR reaction. PCR was performed using HotStar Taq polymerase (QIAGEN). … View Doc

Annexure I List Of Items For Molecular Work All Items Quoted …
9 Hotstar Taq DNA polymerase (Qiagen) 500 U 10 Long PCR Enzyme Mix (Fermentas) (# K0182) 500 U 11 Taq DNA Polymerase (3 Unit/?l) Bangalore Genei 12000 units … Retrieve Full Source

BMC Infectious Diseases BioMed Central
Aag-3'), 50 µM of each dNTP, 0.5 units of HotStar-taq polymerase (Qiagen, Hilden, Germany), and reaction buffer containing 1.5 mM MgCl 2. Cycling-conditions on a … Read Full Source

Simple Sequence Repeat (SSR) Markers For Genetic Mapping Of …
Primers (Qiagen) were used in PCR. The reaction components included 1× PCR buffer with 1.5 mM MgCl2 (Qiagen), 0.67 mM dNTPs, 0.5 µM each primer, 0.4 U HotStar Taq polymerase enzyme … Doc Viewer

Identification And Molecular Characterization Of A Novel …
Follows: 2.5 units HotStar Taq polymerase (Qiagen, Valencia, CA), 13 PCR buffer with 1.5 mM MgCl 2 (Qiagen), 1 ml cDNA, 200 mM dNTPs (deoxynucleoside triphosphates), and 250 ng of primers, us- … Read Full Source

Molecular Analysis Of Archived Blood Slides Reveals An …
Mg/ml acetylated bovine serum albumin (Promega, Madison, Wis.), 0.5 U of HotStar Taq polymerase (Qiagen, Hilden, Germany) and 2.5 µl of sample DNA. … Read Content

Genetic Connectivity In The Florida Reef System: Comparative …
Primer, and 0.75–1.75 U of HotStar Taq DNA Polymerase (QIAGEN Inc.). PCR was performed in a Mastercycler Fig. 1 Map showing individual sponge sampling sites along the Florida reef system. … Return Doc

Overexpression Of Suppressor Of Cytokine Signaling-1 Impairs …
With the Omniscript RT kit (Qiagen) using random hexamers. Polymerase chain reaction (PCR) was performed in a final volume of 25mL containing 1 3PCR buffer (Qiagen), 0.5 mM deoxyribonucleoside triphosphate, 2.5 U HotStar Taq polymerase (Qiagen), and appropriate dilutions of cDNA. … Get Content Here

Strains Of Epstein–Barr Virus Infecting Multiple Sclerosis …
DGTP, and dTTP), 2.5U of HotStar Taq polymerase (Qiagen) and 0.4mM of each primer. Cycling conditions involved an initial 15-min denaturation at 95 C, fol- … Read Document