High Fidelity Taq Polymerase Qiagen

Alternative Promoter Use And Splice Variation In The Human …
(Qiagen, Crawley, UK), according to the manufacturer’s protocol. Purity and concentration of RNA was checked using a Nanodrop ND- In all cases, unless stated, Invitrogen Platinum Taq High Fidelity DNA polymerase was used. … Fetch Here

Protocols For High-Volume DNA Barcode Analysis
B) QIAGEN DNeasy tissue kit (DNeasy 96 tissue kit) by both lowering the DNA melting temperature and stabilizing Taq polymerase (Spiess c) High-fidelity/specificity: e.g. DeepVent Taq (NEB), Diamond Taq (Bioline). … Access Document

HE OURNAL OF IOLOGICAL HEMISTRY © 2004 By The American …
Escherichia coli DNA polymerase I, high fidelity Taq polymerase, calf intestinal alkaline phosphatase, DNase, and T4 DNA ligase were pur- and 2 107 L929 cells using the Qiagen RNeasy Midi kit (Qiagen; Valencia, CA). 10 g of total RNA was treated with 10 units of DNase … View Document

Taq polymerase – Wikipedia, The Free Encyclopedia
Taq polymerase ( / ˌ t æ k ˈ p ɒ l ɨ m ər eɪ z /) is a thermostable DNA polymerase named after the thermophilic bacterium Thermus aquaticus from which it was and archaea, such as Pfu DNA polymerase, possessing a proofreading activity, and are being used instead of (or in combination with) Taq for high-fidelity … Read Article

EXPERIMENT 4 Ð CLONING THE UPSTREAM REGION OF THE GENE OF …
IProof High Fidelity DNA polymerase kit (Cat.# 172-5301, Bio-Rad) Sterile water dNTP mix (2.5 mM each dNTP, from Ex-Taq DNA polymerase package, Takara) QIAgen Plasmid Miniprep kit Nanodrop spectrophotometer TE buffer … Access Content

Directed Evolution Of polymerase Function By …
Thermostability and fidelity, although at the cost of reduced purified by using Ni-NTA Agarose (Qiagen, Chatsworth, CA). Taq polymerase, compartments must remain stable at the high … Read More

Materials And Methods
(iii) For both samples, all purifications steps were done with the MinElute PCR purification kit (Qiagen Pfx (Invitrogen), Herculase II Fusion (Agilent), Pfu Turbo Cx Hotstart (Agilent), Platinum Taq High Fidelity (Invitrogen), AmpliTaq Gold (Applied Biosystems) and Phusion DNA polymerase … Return Doc

On-off Regulation Of 3' Exonuclease Excision To DNA …
Qiagen (Hiden, Germany) PCR purification column according to exonuclease plays an important role in maintaining the high fidelity of exo+ polymerization by Extension of base mispairs by Taq DNA polymerase: … Fetch Document

Contribution Of Bone Marrow-Derived Stem Cells To Endometrium …
Germany, http://www1.qiagen.com). Primers used for sex-deter-mining region of the Y chromosome (SRY) polymerase chain To amplify the 397-base-pair region of SRY, high-fidelity Taq polymerase (Invitrogen, Carlsbad, CA, http://www … Get Doc

A New Class Of Oxidosqualene Cyclases Directs Synthesis Of …
Seedlings by using the RNeasy Plant Mini kit (Qiagen, Chats-worth, CA), and mRNA isolation was carried out by using the (including the 2-kb 5 region) were PCR-amplified with high-fidelity Taq polymerase (Herculase), and 3 independent clones … Content Retrieval

Supporting Information
The RNeasy Plant Mini Kit (Qiagen) from seven-day old whole tracted from leaves (2) as the template, using Apex Taq RED DNA Polymerase Master Mix (Genesee Scientific). Phusion high-fidelity DNA polymerase (Finnzyme). The ampli- … Fetch Content