Half Life Of Taq Polymerase At 94

Introduction
QIAGEN Taq DNA Polymerase used in combination with QIAGEN PCR Buffer provides robust performance for reproducible results in a wide range Half-life: 10 min at 97°C; 60 min at 94°C. 5'–3' exonuclease activity: Yes … Fetch Document

Archaea – Wikipedia, The Free Encyclopedia
Evolutionary history and show many differences in their biochemistry from other forms of life, and This new appreciation of the importance and ubiquity of archaea came from using the polymerase chain irregularly shaped lobed cells in Sulfolobus, needle-like filaments that are less than half a … Read Article

PCR Basics
Cycle Times and Temps Primer Buffer Polymerase Temperature Denaturation Trade off between denaturing DNA and not denaturing Taq Polymerase Taq half-life 40min at 10 μl 10 X PCR buffer 2 μl 200 μM dNTP 1 μl Taq Pol (5 Units/ μl) 93 μl Total Volume Temperature Cycling Denaturation 94 … Fetch Content

Taq DNA Polymerase
The half-life of enzyme is >40 minutes at 95°C. • Taq DNA Polymerase accepts modified nucleotides (e.g. biotin-, digoxigenin-, fluorescent-labeled nucleotides) as Denature the DNA by heating in a thermocycler for 3 minutes at 94°C . 5. Perform ~30 cycles of: … View Doc

Taq PCR Master Mix (2x)
Taq DNA Polymerase Master mix, with stable and reproducible high directly load PCR products to agarose gels. > Taq DNA Polymeraseis a thermostable enzyme of approximately 94 kDa from Thermus aquaticus. > Ultra pure, recombinant protein. > The enzyme replicates DNA at 74°C and exhibits a half-life of 40 … Retrieve Full Source

Taq Plus DNA Polymerase
25-35 cycles 94℃ 55-68℃ 72℃ 30 seconds 30 seconds 1 minute Final extension 72℃ 10 minutes • The half-life of enzyme is >40 minutes at 95°C. • The error rate of Taq Plus DNA Polymerase in PCR is about 1×10-5 errors per nt per cycle; … Visit Document

Time (min) at 94˚C 60 100 80 20 40 0
Time (min) at 94˚C 60 100 80 20 40 0 30 AmpliTaq Gold® DNA Polymerase Taq DNA Polymerase SuperScript™ III gives you: • Reduced RNase H activity for more full-length cDNA (Figure 3) • A half-life of 220 … Read More

X G ENAXXON – DF-Taq DNA-Polymerase
Taq Polymerase is delivered with 10X reaction buffer and separate MgCL2.The enzyme is delivered with our buffer component 'Buffer-S'. Half-life: 20min. at 95°C, 60min. at 94°C 5’-3’ exonuclease activity: Yes … Document Viewer

I Description II Kit Contents …
Month when stored at room temperature (25°C) without significant activity loss.  High PCR yield: The green Taq DNA polymerase has longer enzyme half-life and therefore Initial denaturing: 94°C for three minutes Then 30 cycles of: 94°C for 30 seconds … View Full Source

Extremophile – Wikipedia, The Free Encyclopedia
Temperature range from 30°C to over 94°C and a pH range from 6-10. This catalase is extremely stable compared to other catalases at high temperatures and pH. In a comparative study, the T. brockianus catalase exhibited a half life of 15 DNA modifying enzymes such as Taq DNA polymerase and some … Read Article

HotStarTaq PlusPCR Handbook
Half-life: 10 min at 97°C; 60 min at 94°C 5'–3' exonuclease activity: Yes HotStarTaq PlusDNA Polymerase HotStarTaq Plus DNA Polymerase is a modified form of the recombinant 94 kDa Taq DNA Polymerase from QIAGEN. … Get Doc

X- Chromo DNA Polymerase (recombinant)
• Replicates DNA at 74°C and exhibits a half-life 40 minutes at 95°C. • Generates mostly 3' dA overhang PCR products which are suitable for TA cloning Description X-Chromo Taq DNA Polymerase is a thermostable DNA It is recommended for 10 – 30 sec. at 94°C. The suggested final … Read Content