Go Flexi Taq Polymerase

Ethanologenic Potential Of The Bacterium Bacillus Cereus NB …
Germany) and Promega Go-Taq® Flexi DNA Polymerase (MGW Biotech, Germany). For good practicing no source of DNA was allowed to enter the PCR-room at all. For 20 µl PCR reaction the following reactants were mixed together for a given reaction. … Fetch Content

Germline HVR-II Mitochondrial Polymorphisms Associated With …
DNA and 2 U Taq polymerase (Go Taq® Flexi DNA polymerase). The PCR cycling program comprised an initial denaturation at 95°C for 10 min, followed by 35 cycles of 95°C for 10 s, annealing of primers at 52°C for 30 s and extension at 72°C for 30 s. … Return Doc

The Transcription Factor Nrf2 Is A Therapeutic Target Against …
Taq Flexi Colorless PCR buffer (Promega) containing 0.5 U of Go Taq Flexi DNA polymerase (Promega) and 30 pmol of synthetic gene-specific primers. Primer sequences are shown in Table I. To ensure that equal amounts of cDNA were added to the PCR, the-actin housekeeping gene … Access Full Source

Production And Optimization Of Detergent Compatible …
Biotech, Germany) and Promega Go-Taq® Flexi DNA Polymerase (MGW Biotech, Germany)20. PCR product along with 1Kb ladder from Gib coBRL were then loaded on 0.8% agarose gel in 0.5X Tris-boric acid buffer, containing 1 µl of 10 mg/ml of Ethedium bromide/30 g of … Fetch This Document

SUPPLEMENTAL MATERIAL Supplemental Methods Genotyping Of The …
TM Flexi Buffer, and 0.625U Go TaqTM DNA Polymerase (Promega). Amplifications were carried out in 96well plates on a DNA EngineTM (MJ Research, UK) for 1 cycle of 5 min at 95 oC followed by 30 cycles of 30sec at Flexi Buffer, and 0.625U Go TaqTM DNA Polymerase (Promega). … Access Doc

Phylogenetic Analysis Of Rhizoctonia Solani AG-4 Isolates …
polymerase (Promega, Go-Taq Flexi DNA Polymerase), 2.5 mM dNTP mix, 1X PCR buffer, and 50 pmol of each primer. For amplification, a MWG Primus thermal cycler was used with the following temperature profile: an initial … Fetch Document

New Variable-Number Tandem-Repeat Markers For Typing …
Follows using the Go Taq Flexi DNA polymerase (Promega). Five microliters from fivefold-diluted DNA solution was added to a final volume of 25 l con-taining 0.1 lofGoTaqFlexi DNA polymerase (5 U/ l), 5 l of betaine (Sigma), … Fetch Doc

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SM8301 Promega SM8301 PC PR Go Taq Flexi DNA Polymerase (500U) SM8305 Promega SM8305 PC PR GoTaq Hot Start Green Master Mix (100rxns) SM5122 Promega M5122 PC PR GoTaq Hot Start Polymerase (500u) … Document Viewer

Nuclear And MtDNA Lineage Diversity In Wild And Cultured …
Of Promega Go Taq Flexi polymerase, MgCl 2 specific to each locus, primers ranging from 0.03 to 0.08 lM, depending upon their relative fluorescence in the multiplex, plus water resulting in a final volume of 10 ll. Thermal … Read Full Source

Parasites & Vectors BioMed Central
Taq® DNA polymerase in 5× Go Taq ® Flexi PCR buffer, 1.5 mM MgCl 2 (Promega), 100 ng/μl of each forward and backward primer for each trypanosome species using pro-cedures described by Picozzi et al., [17]. All the 103 DNA … Get Doc